Flow cytometry analyses of adipose tissue macrophages

Kae Won Cho, David L. Morris, Carey N. Lumeng

Research output: Chapter in Book/Report/Conference proceedingChapter

44 Citations (Scopus)

Abstract

Within adipose tissue, multiple leukocyte interactions contribute to metabolic homeostasis in health as well as to the pathogenesis of insulin resistance with obesity. Adipose tissue macrophages (ATMs) are the predominant leukocyte population in fat and contribute to obesity-induced inflammation. Characterization of ATMs and other leukocytes in the stromal vascular fraction from fat has benefited from the use of flow cytometry and flow-assisted cell sorting techniques. These methods permit the immunophenotyping, quantification, and purification of these unique cell populations from multiple adipose tissue depots in rodents and humans. Proper isolation, quantification, and characterization of ATM phenotypes are critical for understanding their role in adipose tissue function and obesity-induced metabolic diseases. Here, we present the flow cytometry protocols for phenotyping ATMs in lean and obese mice employed by our laboratory.

Original languageEnglish (US)
Title of host publicationMethods of Adipose Tissue Biology, Part A
PublisherAcademic Press Inc.
Pages297-314
Number of pages18
Volume537
ISBN (Print)9780124116191
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume537
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Flow cytometry
Macrophages
Adipose Tissue
Flow Cytometry
Tissue
Leukocytes
Obesity
Fats
Cells
Obese Mice
Immunophenotyping
Metabolic Diseases
Sorting
Population
Purification
Blood Vessels
Insulin Resistance
Rodentia
Homeostasis
Health

Keywords

  • Adipose tissue macrophages
  • Flow cytometry
  • Stromal vascular cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Cho, K. W., Morris, D. L., & Lumeng, C. N. (2014). Flow cytometry analyses of adipose tissue macrophages. In Methods of Adipose Tissue Biology, Part A (Vol. 537, pp. 297-314). (Methods in Enzymology; Vol. 537). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-411619-1.00016-1

Flow cytometry analyses of adipose tissue macrophages. / Cho, Kae Won; Morris, David L.; Lumeng, Carey N.

Methods of Adipose Tissue Biology, Part A. Vol. 537 Academic Press Inc., 2014. p. 297-314 (Methods in Enzymology; Vol. 537).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cho, KW, Morris, DL & Lumeng, CN 2014, Flow cytometry analyses of adipose tissue macrophages. in Methods of Adipose Tissue Biology, Part A. vol. 537, Methods in Enzymology, vol. 537, Academic Press Inc., pp. 297-314. https://doi.org/10.1016/B978-0-12-411619-1.00016-1
Cho KW, Morris DL, Lumeng CN. Flow cytometry analyses of adipose tissue macrophages. In Methods of Adipose Tissue Biology, Part A. Vol. 537. Academic Press Inc. 2014. p. 297-314. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-411619-1.00016-1
Cho, Kae Won ; Morris, David L. ; Lumeng, Carey N. / Flow cytometry analyses of adipose tissue macrophages. Methods of Adipose Tissue Biology, Part A. Vol. 537 Academic Press Inc., 2014. pp. 297-314 (Methods in Enzymology).
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