Flow cytometry analyses of adipose tissue macrophages

Kae Won Cho, David L. Morris, Carey N. Lumeng

Research output: Chapter in Book/Report/Conference proceedingChapter

51 Scopus citations

Abstract

Within adipose tissue, multiple leukocyte interactions contribute to metabolic homeostasis in health as well as to the pathogenesis of insulin resistance with obesity. Adipose tissue macrophages (ATMs) are the predominant leukocyte population in fat and contribute to obesity-induced inflammation. Characterization of ATMs and other leukocytes in the stromal vascular fraction from fat has benefited from the use of flow cytometry and flow-assisted cell sorting techniques. These methods permit the immunophenotyping, quantification, and purification of these unique cell populations from multiple adipose tissue depots in rodents and humans. Proper isolation, quantification, and characterization of ATM phenotypes are critical for understanding their role in adipose tissue function and obesity-induced metabolic diseases. Here, we present the flow cytometry protocols for phenotyping ATMs in lean and obese mice employed by our laboratory.

Original languageEnglish (US)
Title of host publicationMethods of Adipose Tissue Biology, Part A
PublisherAcademic Press Inc.
Pages297-314
Number of pages18
ISBN (Print)9780124116191
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Enzymology
Volume537
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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Keywords

  • Adipose tissue macrophages
  • Flow cytometry
  • Stromal vascular cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Cho, K. W., Morris, D. L., & Lumeng, C. N. (2014). Flow cytometry analyses of adipose tissue macrophages. In Methods of Adipose Tissue Biology, Part A (pp. 297-314). (Methods in Enzymology; Vol. 537). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-411619-1.00016-1