Flt3 ligand induces tyrosine phosphorylation of Gab1 and Gab2 and their association with Shp-2, Grb2, and PI3 kinase

Shuli Zhang, Hal Broxmeyer

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The receptor tyrosine kinase Flt3 has been shown to play an important role in proliferation, differentiation, and survival of hematopoietic stem and progenitor cells. Although some postreceptor signaling events of Flt3 have been characterized, the involvement of Gab family proteins in Flt3 signaling is not known. In this study, we show that both Gab1 and Gab2 are rapidly tyrosine phosphorylated after Flt3 ligand stimulation of Flt3 ligand-responsive cells. They interact with tyrosine-phosphorylated Shp-2, p85, Grb2, and Shc. The results suggest that Gab proteins are engaged in Flt3 signaling to mediate downstream activation of Shp-2 and PI3 kinase pathways and possibly the Ras/Raf/MAPK pathway. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume277
Issue number1
DOIs
StatePublished - Oct 14 2000

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Phosphorylation
Hematopoietic Stem Cells
Phosphatidylinositol 3-Kinases
Tyrosine
Association reactions
Receptor Protein-Tyrosine Kinases
Proteins
Chemical activation
flt3 ligand protein

Keywords

  • Flt3
  • Gab
  • Hematopoietic cells
  • Signal transduction
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AB - The receptor tyrosine kinase Flt3 has been shown to play an important role in proliferation, differentiation, and survival of hematopoietic stem and progenitor cells. Although some postreceptor signaling events of Flt3 have been characterized, the involvement of Gab family proteins in Flt3 signaling is not known. In this study, we show that both Gab1 and Gab2 are rapidly tyrosine phosphorylated after Flt3 ligand stimulation of Flt3 ligand-responsive cells. They interact with tyrosine-phosphorylated Shp-2, p85, Grb2, and Shc. The results suggest that Gab proteins are engaged in Flt3 signaling to mediate downstream activation of Shp-2 and PI3 kinase pathways and possibly the Ras/Raf/MAPK pathway. (C) 2000 Academic Press.

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