Flt3-ligand production by human bone marrow stromal cells

M. Lisovsky, S. E. Braun, Y. Ge, H. Takahira, L. Lu, V. G. Savchenko, S. D. Lyman, H. E. Broxmeyer

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Abstract

Bone marrow stromal cells are important sources of cytokines and growth factors which participate in regulation of proliferation and differentiation of hematopoietic stem and progenitor cells. Recently flt3/flk-2-ligand (flt3-L), a new growth factor which uses a membrane tyrosine kinase receptor, was cloned. It is expressed in transmembrane and soluble forms and stimulates/co-stimulates proliferation and colony formation of hematopoietic stem/progenitor cells. It has not been reported whether flt3-L is produced by cells of the hematopoietic bone marrow microenvironment. We demonstrate the expression of flt3-L in bone marrow fibroblasts (BMF) and in stromal cells of adherent layers of long-term bone marrow cultures by RT-PCR, Northern blot, immunocytochemistry and FAGS analysis. The latter two methods localized flt3-L intracellularly and on cell membranes. Treatment with interleukin-1α increased the expression of flt3-L in BMF. This demonstrates production and modulation of flt3-L in stromal cells of human bone marrow.

Original languageEnglish (US)
Pages (from-to)1012-1018
Number of pages7
JournalLeukemia
Volume10
Issue number6
StatePublished - Jun 1996

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Keywords

  • Bone marrow microenvironment
  • flt3-ligand
  • Interleukin 1
  • Stromal cells

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Lisovsky, M., Braun, S. E., Ge, Y., Takahira, H., Lu, L., Savchenko, V. G., Lyman, S. D., & Broxmeyer, H. E. (1996). Flt3-ligand production by human bone marrow stromal cells. Leukemia, 10(6), 1012-1018.