Flt3-ligand production by human bone marrow stromal cells

M. Lisovsky, S. E. Braun, Y. Ge, H. Takahira, L. Lu, V. G. Savchenko, S. D. Lyman, H. E. Broxmeyer

Research output: Contribution to journalArticle

87 Scopus citations


Bone marrow stromal cells are important sources of cytokines and growth factors which participate in regulation of proliferation and differentiation of hematopoietic stem and progenitor cells. Recently flt3/flk-2-ligand (flt3-L), a new growth factor which uses a membrane tyrosine kinase receptor, was cloned. It is expressed in transmembrane and soluble forms and stimulates/co-stimulates proliferation and colony formation of hematopoietic stem/progenitor cells. It has not been reported whether flt3-L is produced by cells of the hematopoietic bone marrow microenvironment. We demonstrate the expression of flt3-L in bone marrow fibroblasts (BMF) and in stromal cells of adherent layers of long-term bone marrow cultures by RT-PCR, Northern blot, immunocytochemistry and FAGS analysis. The latter two methods localized flt3-L intracellularly and on cell membranes. Treatment with interleukin-1α increased the expression of flt3-L in BMF. This demonstrates production and modulation of flt3-L in stromal cells of human bone marrow.

Original languageEnglish (US)
Pages (from-to)1012-1018
Number of pages7
Issue number6
StatePublished - Jun 1996


  • Bone marrow microenvironment
  • flt3-ligand
  • Interleukin 1
  • Stromal cells

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

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  • Cite this

    Lisovsky, M., Braun, S. E., Ge, Y., Takahira, H., Lu, L., Savchenko, V. G., Lyman, S. D., & Broxmeyer, H. E. (1996). Flt3-ligand production by human bone marrow stromal cells. Leukemia, 10(6), 1012-1018.