Fluorescence in situ hybridization (fish) detection of chromosomal 12p anomalies in testicular germ cell tumors

Liang Cheng, Darrell D. Davidson, Rodolfo Montironi, Mingsheng Wang, Antonio Lopez-Beltran, Timothy A. Masterson, Costantine Albany, Shaobo Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gains of genetic material or internal rearrangements of chromosome 12p, including 12p overrepresentation or isochromosome 12p [i(12p)], are observed in virtually all germ cell tumors (GCT), in all histologic subtypes, and from various body locations. The chromosomal region involved in these alterations contains the growth and survival promoting oncogene KRAS (12p12.1). Gains or rearrangements of 12p characterize GCT from in situ to chemoresistant stages. Fluorescence in situ hybridization (FISH) detection of chromosome 12p anomalies is a sensitive and specific test for the diagnosis of germ cell tumors. Here we provide a detailed protocol for FISH detection of isochromosome 12p and chromosome 12p overrepresentation. The method is helpful for diagnosis of germ cell origin, and for selection of patients who may benefit from cisplatin-based chemotherapy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages49-63
Number of pages15
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2195
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Differential diagnosis
  • Fluorescence in situ hybridization (FISH)
  • Germ cell tumor
  • Isochromosome 12p/chromosomal 12p anomalies
  • Testis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Cheng, L., Davidson, D. D., Montironi, R., Wang, M., Lopez-Beltran, A., Masterson, T. A., Albany, C., & Zhang, S. (2021). Fluorescence in situ hybridization (fish) detection of chromosomal 12p anomalies in testicular germ cell tumors. In Methods in Molecular Biology (pp. 49-63). (Methods in Molecular Biology; Vol. 2195). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0860-9_4