Fluorescent bone viewed through toenails of living animals: A method to observe bone regrowth

D. A. Neufeld, Khalid Mohammad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have developed a new method to observe bone and to document growth in living animals. The technique involves injecting calcein, a fluorescent calcium deposition marker, waiting approximately 4 hr for it to clear the vascular system, and observing bone directly through the toenails of lightly anesthetized living animals. Bone regrowth can be monitored in situ by amputating the digit through the nail plate, waiting the desired number of days, and injecting a second fluorescent label, alizarin red. Bone that has regrown since the amputation appears as a red distal to the green calcein label on toes of lightly anesthetized animals when viewed under FITC fluorescence. This method has been used to demonstrate blocked bone synthesis and to quantitate significant differences in bone growth in control and experimental toes of individual animals. Advantages of this method include its simplicity, the use of fewer animals to collect sequential data, and increased reliability of repeated microscopic measurements using the same animal.

Original languageEnglish (US)
Pages (from-to)259-263
Number of pages5
JournalBiotechnic and Histochemistry
Volume75
Issue number6
StatePublished - 2000
Externally publishedYes

Fingerprint

Nails
Bone and Bones
Toes
Fluorescein-5-isothiocyanate
Bone Development
Amputation
Blood Vessels
Fluorescence
Calcium
Growth

Keywords

  • Amputation
  • Bone growth
  • Bone regeneration
  • Fluorescent labels
  • Quantitation

ASJC Scopus subject areas

  • Anatomy
  • Applied Microbiology and Biotechnology

Cite this

Fluorescent bone viewed through toenails of living animals : A method to observe bone regrowth. / Neufeld, D. A.; Mohammad, Khalid.

In: Biotechnic and Histochemistry, Vol. 75, No. 6, 2000, p. 259-263.

Research output: Contribution to journalArticle

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