Fluorescent protein tools for studying protein dynamics in living cells

A review

Richard Day, Fred Schaufele

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have witnessed remarkable advances over the past decade in the application of optical techniques to visualize the genetically encoded fluorescent proteins (FPs) in living systems. The imaging of the FPs inside living cells has become an essential tool for studies of cell biology and physiology. FPs are now available that span the visible spectrum from deep blue to deep red, providing a wide choice of genetically encoded fluorescent markers. Furthermore, some FPs have been identified that have unusual characteristics that make them useful reporters of the dynamic behaviors of proteins inside cells. These additions to the FP toolbox are now being used for some very innovative live-cell imaging applications. Here, we will highlight the characteristics and uses of a few of these exceptional probes. Many different optical methods can be combined with the FPs from marine organisms to provide quantitative measurements in living systems.

Original languageEnglish (US)
Article number031202
JournalJournal of Biomedical Optics
Volume13
Issue number3
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Cells
proteins
Proteins
cytology
Cytology
Imaging techniques
Cell Physiological Phenomena
Aquatic Organisms
Physiology
visible spectrum
cells
organisms
markers
Cell Biology
optics
probes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Fluorescent protein tools for studying protein dynamics in living cells : A review. / Day, Richard; Schaufele, Fred.

In: Journal of Biomedical Optics, Vol. 13, No. 3, 031202, 2008.

Research output: Contribution to journalArticle

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