Fluoride Dentifrice Overcomes the Lower Resistance of Fluorotic Enamel to Demineralization

Luma Fabiane Almeida, Lina María Marín, Esperanza Angeles Martínez-Mier, Jaime Aparecido Cury

Research output: Contribution to journalArticlepeer-review


We evaluated if the low resistance of fluorotic enamel to demineralization could be overcome by fluoride dentifrice (FD) treatment. Paired enamel slabs of sound and fluorotic enamel (n = 20/group) from human teeth presenting Thylstrup and Fejerskov index (TF) scores from 0 to 4 were obtained. Half of the anatomic surface of the enamel slabs was isolated and used as a control (baseline) regarding enamel mineralization and fluoride concentration. The slabs were submitted to a pH-cycling model simulating a high cariogenic challenge, and 2×/day they were treated with placebo dentifrice (PD) or FD (1,100 μg F/g, as NaF). After 10 days, the slabs were cut into two halves. Enamel demineralization was evaluated by cross-sectional microhardness in one half, and the fluoride formed (FF) concentration was determined in the other half. For statistical analysis, the data on net demineralization area (ΔΔS) and FF (μg F/g) were grouped into TF0, TF1-2, and TF3-4, and analyzed by two-way ANOVA followed by Tukey's test (α = 5%). The factors studied were TF (0, 1-2, and 3-4) and dentifrice treatment (PD or FD). The effect of the factors was statistically significant for ΔΔS and FF (p < 0.05). In the PD group, the following pattern for ΔΔS was observed: TF3-4 > TF1-2 > TF0 (p < 0.05); however, the groups did not differ (p > 0.05) when FD was used. Regarding FF, the groups treated with PD did not differ (p > 0.05), but the greatest (p < 0.05) FF concentration was found in group TF3-4 treated with FD. These findings suggest that the higher susceptibility of fluorotic enamel to demineralization lesions is decreased by the use of FD.

Original languageEnglish (US)
Pages (from-to)567-575
Number of pages9
JournalCaries research
Issue number5
StatePublished - Jul 1 2019


  • Dental caries
  • Dental fluorosis
  • Dentifrice
  • Fluoride
  • Hypomineralization

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of 'Fluoride Dentifrice Overcomes the Lower Resistance of Fluorotic Enamel to Demineralization'. Together they form a unique fingerprint.

Cite this