Fluorometric determination of total phospholipids in rat tissues

Robert A. Harris, David Gambal

Research output: Contribution to journalArticle

13 Scopus citations


A simple, rapid, and sensitive fluorometric procedure for the quantitative determination of total phospholipids in lipid extracts of rat tissue and for monitoring column eluates for phospholipids is presented. It is based on the fluorescence of the phospholipid-rhodamine 6G complex formed by interacting the acidic phosphate group with the basic dye, and is specific for phospholipids. Cholesterol or neutral lipids from tissues do not affect the fluorescence in any manner. Standard curves of mixed phospholipids are prepared for each tissue investigated because each tissue has a different phospholipid composition and therefore a different fluorescent intensity. Total phospholipids were determined by the chemical, chromatographic, and fluorometric procedures and the results compared favorably.

Original languageEnglish (US)
Pages (from-to)479-488
Number of pages10
JournalAnalytical biochemistry
Issue number6
StatePublished - Jun 1963
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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