Follicular helper T cells mediate IgE antibody response to airborne allergens

Takao Kobayashi, Koji Iijima, Alexander Dent, Hirohito Kita

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background TH2 cells have long been believed to play a pivotal role in allergic immune responses, including IgE antibody production and type 2 cytokine–mediated inflammation and pathology. A new T-cell subset, follicular helper T (TFH) cells, is specialized in supporting B-cell maturation and antibody production. Objective We sought to investigate the roles of TFH cells in allergic immune responses. Methods Naive mice were exposed to cytokines or natural allergens through the airways. Development of allergic immune responses was analyzed by collecting draining lymph nodes and sera and by challenging the animals. Cytokine reporter mice and gene-deficient mice were used to dissect the immunologic mechanisms. Results We observed the development of IL-4–producing TFH cells and TH2 cells in draining lymph nodes after airway exposure to IL-1 family cytokines or natural allergens. TFH and TH2 cells demonstrated unique phenotypes, tissue localization, and cytokine responses. TFH cells supported the sustained production of IgE antibody in vivo in the absence of other T-cell subsets or even when TH2 cell functions were severely compromised. Conversely, conditional deficiency of the master regulator Bcl6 in CD4+ T cells resulted in a marked reduction in TFH cell numbers and IgE antibody levels, but type 2 cytokine responses and eosinophilic inflammation in the airways remained unaffected. Conclusion TFH cells play critical roles in the regulation of IgE antibody production. Allergic immune responses to airborne allergens likely involve 2 distinct subsets of IL-4–producing CD4+ T cells, namely TFH and Th2 cells.

Original languageEnglish (US)
Pages (from-to)300-313.e7
JournalJournal of Allergy and Clinical Immunology
Volume139
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Helper-Inducer T-Lymphocytes
Allergens
Immunoglobulin E
Antibody Formation
Cytokines
T-Lymphocyte Subsets
Lymph Nodes
Inflammation
Th2 Cells
Interleukin-1
Reporter Genes
B-Lymphocytes
Cell Count
Pathology
Phenotype
Antibodies
Serum

Keywords

  • allergens
  • allergy
  • Follicular T cells
  • IgE
  • IL-4
  • T2 cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Follicular helper T cells mediate IgE antibody response to airborne allergens. / Kobayashi, Takao; Iijima, Koji; Dent, Alexander; Kita, Hirohito.

In: Journal of Allergy and Clinical Immunology, Vol. 139, No. 1, 01.01.2017, p. 300-313.e7.

Research output: Contribution to journalArticle

Kobayashi, Takao ; Iijima, Koji ; Dent, Alexander ; Kita, Hirohito. / Follicular helper T cells mediate IgE antibody response to airborne allergens. In: Journal of Allergy and Clinical Immunology. 2017 ; Vol. 139, No. 1. pp. 300-313.e7.
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