Footprint analysis of replicating murine leukemia virus reverse transcriptase

Birgitta M. Wöhrl, Millie M. Georgiadis, Alice Telesnitsky, Wayne A. Hendrickson, Stuart F.J. Le Grice

Research output: Contribution to journalArticle

50 Scopus citations


Replication complexes that contained either murine leukemia virus reverse transcriptase (MLV RT) or a variant reverse transcriptase without a ribonuclease (RNase) H domain (ΔRH MLV RT ) were visualized by enzymatic footprinting. Wild-type MLV RT protected template nucleotides +6 to -27, and primer nucleotides -1 to -26 of primers that had first been extended by one or four nucleotides. Although it catalyzed DNA synthesis, ARH MLV RT stably bound template-primer only under conditions of reduced ionic strength and protected the duplex portion only as far as position -15. Despite altered hydrolysis profiles, both enzymes covered primarily the template-primer duplex, contradicting recent predictions based on the structure of rat DNA polymerase β.

Original languageEnglish (US)
Pages (from-to)96-99
Number of pages4
Issue number5194
StatePublished - Jan 1 1995


ASJC Scopus subject areas

  • General

Cite this

Wöhrl, B. M., Georgiadis, M. M., Telesnitsky, A., Hendrickson, W. A., & Le Grice, S. F. J. (1995). Footprint analysis of replicating murine leukemia virus reverse transcriptase. Science, 267(5194), 96-99.