Footprint analysis of replicating murine leukemia virus reverse transcriptase

Birgitta M. Wöhrl, Millie Georgiadis, Alice Telesnitsky, Wayne A. Hendrickson, Stuart F J Le Grice

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Replication complexes that contained either murine leukemia virus reverse transcriptase (MLV RT) or a variant reverse transcriptase without a ribonuclease (RNase) H domain (ΔRH MLV RT ) were visualized by enzymatic footprinting. Wild-type MLV RT protected template nucleotides +6 to -27, and primer nucleotides -1 to -26 of primers that had first been extended by one or four nucleotides. Although it catalyzed DNA synthesis, ARH MLV RT stably bound template-primer only under conditions of reduced ionic strength and protected the duplex portion only as far as position -15. Despite altered hydrolysis profiles, both enzymes covered primarily the template-primer duplex, contradicting recent predictions based on the structure of rat DNA polymerase β.

Original languageEnglish
Pages (from-to)96-99
Number of pages4
JournalScience
Volume267
Issue number5195
StatePublished - Jan 6 1995
Externally publishedYes

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Murine Leukemia Viruses
RNA-Directed DNA Polymerase
Nucleotides
Ribonuclease H
DNA-Directed DNA Polymerase
Osmolar Concentration
Hydrolysis
DNA
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Wöhrl, B. M., Georgiadis, M., Telesnitsky, A., Hendrickson, W. A., & Le Grice, S. F. J. (1995). Footprint analysis of replicating murine leukemia virus reverse transcriptase. Science, 267(5195), 96-99.

Footprint analysis of replicating murine leukemia virus reverse transcriptase. / Wöhrl, Birgitta M.; Georgiadis, Millie; Telesnitsky, Alice; Hendrickson, Wayne A.; Le Grice, Stuart F J.

In: Science, Vol. 267, No. 5195, 06.01.1995, p. 96-99.

Research output: Contribution to journalArticle

Wöhrl, BM, Georgiadis, M, Telesnitsky, A, Hendrickson, WA & Le Grice, SFJ 1995, 'Footprint analysis of replicating murine leukemia virus reverse transcriptase', Science, vol. 267, no. 5195, pp. 96-99.
Wöhrl BM, Georgiadis M, Telesnitsky A, Hendrickson WA, Le Grice SFJ. Footprint analysis of replicating murine leukemia virus reverse transcriptase. Science. 1995 Jan 6;267(5195):96-99.
Wöhrl, Birgitta M. ; Georgiadis, Millie ; Telesnitsky, Alice ; Hendrickson, Wayne A. ; Le Grice, Stuart F J. / Footprint analysis of replicating murine leukemia virus reverse transcriptase. In: Science. 1995 ; Vol. 267, No. 5195. pp. 96-99.
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