Forced expression of Genesis, a winged helix transcriptional repressor isolated from embryonic stem cells, blocks granulocytic differentiation of 32D myeloid cells

D. Xu, Mervin Yoder, J. Sutton, R. Hromas

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We recently isolated and characterized a novel member of the winged helix (formerly HNF-3/Forkhead) transcriptional regulatory family, termed Genesis. Genesis was found to be a transcriptional repressor expressed almost exclusively in embryonic stem cells or embryonal carcinoma cells. This expression rapidly declined when these cells were stimulated to differentiate. This expression pattern suggested that Genesis may play a role in cellular differentiation. To explore that possibility in a heterologous system of differentiation, Genesis was stably transduced into the IL-3-dependent myeloid cell line 32D using a retroviral expression vector. 32D cells overexpressing Genesis failed to mature normally when stimulated with G-CSF but continued to proliferate and maintained a primitive phenotype. This finding implicates Genesis in the regulation of development, and also implies that there may be common transcription pathways for many developing tissues.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalLeukemia
Volume12
Issue number2
DOIs
StatePublished - 1998

Fingerprint

Myeloid Cells
Embryonic Stem Cells
Embryonal Carcinoma Stem Cells
Interleukin-3
Granulocyte Colony-Stimulating Factor
Phenotype
Cell Line

Keywords

  • Differentiation
  • Embryonal carcinoma
  • Embryonic stem cells
  • Myeloid cells
  • Transcription factors

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Forced expression of Genesis, a winged helix transcriptional repressor isolated from embryonic stem cells, blocks granulocytic differentiation of 32D myeloid cells. / Xu, D.; Yoder, Mervin; Sutton, J.; Hromas, R.

In: Leukemia, Vol. 12, No. 2, 1998, p. 207-212.

Research output: Contribution to journalArticle

@article{00e582f968e24c488b62e00e0c8aa88b,
title = "Forced expression of Genesis, a winged helix transcriptional repressor isolated from embryonic stem cells, blocks granulocytic differentiation of 32D myeloid cells",
abstract = "We recently isolated and characterized a novel member of the winged helix (formerly HNF-3/Forkhead) transcriptional regulatory family, termed Genesis. Genesis was found to be a transcriptional repressor expressed almost exclusively in embryonic stem cells or embryonal carcinoma cells. This expression rapidly declined when these cells were stimulated to differentiate. This expression pattern suggested that Genesis may play a role in cellular differentiation. To explore that possibility in a heterologous system of differentiation, Genesis was stably transduced into the IL-3-dependent myeloid cell line 32D using a retroviral expression vector. 32D cells overexpressing Genesis failed to mature normally when stimulated with G-CSF but continued to proliferate and maintained a primitive phenotype. This finding implicates Genesis in the regulation of development, and also implies that there may be common transcription pathways for many developing tissues.",
keywords = "Differentiation, Embryonal carcinoma, Embryonic stem cells, Myeloid cells, Transcription factors",
author = "D. Xu and Mervin Yoder and J. Sutton and R. Hromas",
year = "1998",
doi = "10.1038/sj.leu.2400948",
language = "English",
volume = "12",
pages = "207--212",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Forced expression of Genesis, a winged helix transcriptional repressor isolated from embryonic stem cells, blocks granulocytic differentiation of 32D myeloid cells

AU - Xu, D.

AU - Yoder, Mervin

AU - Sutton, J.

AU - Hromas, R.

PY - 1998

Y1 - 1998

N2 - We recently isolated and characterized a novel member of the winged helix (formerly HNF-3/Forkhead) transcriptional regulatory family, termed Genesis. Genesis was found to be a transcriptional repressor expressed almost exclusively in embryonic stem cells or embryonal carcinoma cells. This expression rapidly declined when these cells were stimulated to differentiate. This expression pattern suggested that Genesis may play a role in cellular differentiation. To explore that possibility in a heterologous system of differentiation, Genesis was stably transduced into the IL-3-dependent myeloid cell line 32D using a retroviral expression vector. 32D cells overexpressing Genesis failed to mature normally when stimulated with G-CSF but continued to proliferate and maintained a primitive phenotype. This finding implicates Genesis in the regulation of development, and also implies that there may be common transcription pathways for many developing tissues.

AB - We recently isolated and characterized a novel member of the winged helix (formerly HNF-3/Forkhead) transcriptional regulatory family, termed Genesis. Genesis was found to be a transcriptional repressor expressed almost exclusively in embryonic stem cells or embryonal carcinoma cells. This expression rapidly declined when these cells were stimulated to differentiate. This expression pattern suggested that Genesis may play a role in cellular differentiation. To explore that possibility in a heterologous system of differentiation, Genesis was stably transduced into the IL-3-dependent myeloid cell line 32D using a retroviral expression vector. 32D cells overexpressing Genesis failed to mature normally when stimulated with G-CSF but continued to proliferate and maintained a primitive phenotype. This finding implicates Genesis in the regulation of development, and also implies that there may be common transcription pathways for many developing tissues.

KW - Differentiation

KW - Embryonal carcinoma

KW - Embryonic stem cells

KW - Myeloid cells

KW - Transcription factors

UR - http://www.scopus.com/inward/record.url?scp=0031908809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031908809&partnerID=8YFLogxK

U2 - 10.1038/sj.leu.2400948

DO - 10.1038/sj.leu.2400948

M3 - Article

C2 - 9519783

AN - SCOPUS:0031908809

VL - 12

SP - 207

EP - 212

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 2

ER -