Formation of multinucleated cells that respond to osteotropic hormones in long term human bone marrow cultures

B. R. MacDonald, N. Takahashi, L. M. McManus, J. Holahan, G. R. Mundy, G. David Roodman

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Studies of osteoclasts and their precursors in normal and pathological states have been severely hampered by the lack of an in vitro system for forming osteoclasts. We developed a human marrow culture system in which multinucleated cells with several characteristics of osteoclasts form. Multinucleated cells began to form during the first week of culture, with maximum numbers formed after 3 weeks. PTH (25-50 ng/ml) and 1,25-dihydroxyvitamin D3 (10-10-10-8 M) increased formation of these cells, and these effects were inhibited by calcitonin. These multinucleated cells contained nonspecific esterase and tartrate-resistant acid phosphatase, a marked enzyme of osteoclasts, and had several ultrastructural features of osteoclasts. We used this marrow cell culture technique to study a patient with hyperparathyroidism and markedly increased osteoclasts on bone marrow biopsy. The marrow from this patient formed increased numbers of multinucleated cells in vitro. After parathyroidectomy both multinucleated cell formation in vitro and osteoclast numbers on bone biopsy decreased significantly. This long term marrow culture system represents the first demonstration of human osteoclast-like cell formation in vitro. This system should permit studies to evaluate factors controlling formation of cells with certain osteoclast characteristics in vitro and their precursors as well as to evaluate abnormalities in osteoclast formation in patients with metabolic bone disease.

Original languageEnglish
Pages (from-to)2326-2336
Number of pages11
JournalEndocrinology
Volume120
Issue number6
StatePublished - 1987
Externally publishedYes

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Osteoclasts
Bone Marrow
Hormones
Biopsy
Parathyroidectomy
Carboxylesterase
Hyperparathyroidism
Calcitriol
Metabolic Bone Diseases
Calcitonin
Cell Culture Techniques
Cell Count
In Vitro Techniques
Bone and Bones

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

MacDonald, B. R., Takahashi, N., McManus, L. M., Holahan, J., Mundy, G. R., & Roodman, G. D. (1987). Formation of multinucleated cells that respond to osteotropic hormones in long term human bone marrow cultures. Endocrinology, 120(6), 2326-2336.

Formation of multinucleated cells that respond to osteotropic hormones in long term human bone marrow cultures. / MacDonald, B. R.; Takahashi, N.; McManus, L. M.; Holahan, J.; Mundy, G. R.; Roodman, G. David.

In: Endocrinology, Vol. 120, No. 6, 1987, p. 2326-2336.

Research output: Contribution to journalArticle

MacDonald, BR, Takahashi, N, McManus, LM, Holahan, J, Mundy, GR & Roodman, GD 1987, 'Formation of multinucleated cells that respond to osteotropic hormones in long term human bone marrow cultures', Endocrinology, vol. 120, no. 6, pp. 2326-2336.
MacDonald, B. R. ; Takahashi, N. ; McManus, L. M. ; Holahan, J. ; Mundy, G. R. ; Roodman, G. David. / Formation of multinucleated cells that respond to osteotropic hormones in long term human bone marrow cultures. In: Endocrinology. 1987 ; Vol. 120, No. 6. pp. 2326-2336.
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