Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry

Elitza S. Theel, Bryan H. Schmitt, Leslie Hall, Scott A. Cunningham, Robert C. Walchak, Robin Patel, Nancy L. Wengenack

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

An on-plate testing method using formic acid was evaluated on the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry system using 90 yeast and 78 Corynebacterium species isolates, and 95.6 and 81.1% of yeast and 96.1 and 92.3% of Corynebacterium isolates were correctly identified to the genus and species levels, respectively. The on-plate method using formic acid yielded identification percentages similar to those for the conventional but more laborious tube-based extraction.

Original languageEnglish (US)
Pages (from-to)3093-3095
Number of pages3
JournalJournal of clinical microbiology
Volume50
Issue number9
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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