Forming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression

Renukaradhya J. Gourapura, Masood A. Khan, Richard M. Gallo, Daniel Shaji, Jianyun Liu, Randy R. Brutkiewicz

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

CD1d molecules are structurally similar to MHC class I, but present lipid antigens as opposed to peptides. Here, we show that MHC class I molecules physically associate with (and regulate the functional expression of) mouse CD1d on the surface of cells. Low pH (3.0) acid stripping of MHC class I molecules resulted in increased surface expression of murine CD1d on antigen presenting cells as well as augmented CD1d-mediated antigen presentation to NKT cells. Consistent with the above results, TAP1-/- mice were found to have a higher percentage of type I NKT cells as compared to wild type mice. Moreover, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD1d compared to wild type mice. Together, these results suggest that MHC class I molecules can regulate NKT cell function, in part, by masking CD1d.

Original languageEnglish (US)
Article numbere72867
JournalPLoS ONE
Volume8
Issue number8
DOIs
StatePublished - Aug 29 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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