Forming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression

Renukaradhya J. Gourapura, Masood A. Khan, Richard M. Gallo, Daniel Shaji, Jianyun Liu, Randy Brutkiewicz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

CD1d molecules are structurally similar to MHC class I, but present lipid antigens as opposed to peptides. Here, we show that MHC class I molecules physically associate with (and regulate the functional expression of) mouse CD1d on the surface of cells. Low pH (3.0) acid stripping of MHC class I molecules resulted in increased surface expression of murine CD1d on antigen presenting cells as well as augmented CD1d-mediated antigen presentation to NKT cells. Consistent with the above results, TAP1-/- mice were found to have a higher percentage of type I NKT cells as compared to wild type mice. Moreover, bone marrow-derived dendritic cells from TAP1-/- mice showed increased antigen presentation by CD1d compared to wild type mice. Together, these results suggest that MHC class I molecules can regulate NKT cell function, in part, by masking CD1d.

Original languageEnglish
Article numbere72867
JournalPLoS One
Volume8
Issue number8
DOIs
StatePublished - Aug 29 2013

Fingerprint

CD1d Antigen
Natural Killer T-Cells
Molecules
mice
Antigen Presentation
Antigens
antigen presentation
Bone
cells
Antigen-Presenting Cells
Peptides
Acids
antigen-presenting cells
Dendritic Cells
dendritic cells
bone marrow
Bone Marrow
peptides
antigens
acids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Forming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression. / Gourapura, Renukaradhya J.; Khan, Masood A.; Gallo, Richard M.; Shaji, Daniel; Liu, Jianyun; Brutkiewicz, Randy.

In: PLoS One, Vol. 8, No. 8, e72867, 29.08.2013.

Research output: Contribution to journalArticle

Gourapura, Renukaradhya J. ; Khan, Masood A. ; Gallo, Richard M. ; Shaji, Daniel ; Liu, Jianyun ; Brutkiewicz, Randy. / Forming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression. In: PLoS One. 2013 ; Vol. 8, No. 8.
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