Four Dimensional Image Registration for Intravital Microscopy

Chichen Fu, Neeraj Gadgil, Khalid K. Tahboub, Paul Salama, Kenneth Dunn, Edward J. Delp

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Increasingly the behavior of living systems is being evaluated using intravital microscopy since it provides subcellular resolution of biological processes in an intact living organism. Intravital microscopy images are frequently confounded by motion resulting from animal respiration and heartbeat. In this paper we describe an image registration method capable of correcting motion artifacts in three dimensional fluorescence microscopy images collected over time. Our method uses 3D B-Spline non-rigid registration using a coarse-to-fine strategy to register stacks of images collected at different time intervals and 4D rigid registration to register 3D volumes over time. The results show that our proposed method has the ability of correcting global motion artifacts of sample tissues in four dimensional space, thereby revealing the motility of individual cells in the tissue.

Original languageEnglish (US)
Title of host publicationProceedings - 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016
PublisherIEEE Computer Society
Pages1390-1398
Number of pages9
ISBN (Electronic)9781467388504
DOIs
StatePublished - Dec 16 2016
Event29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016 - Las Vegas, United States
Duration: Jun 26 2016Jul 1 2016

Other

Other29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016
CountryUnited States
CityLas Vegas
Period6/26/167/1/16

Fingerprint

Image registration
Tissue
Fluorescence microscopy
Splines
Animals
Intravital Microscopy

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Electrical and Electronic Engineering

Cite this

Fu, C., Gadgil, N., Tahboub, K. K., Salama, P., Dunn, K., & Delp, E. J. (2016). Four Dimensional Image Registration for Intravital Microscopy. In Proceedings - 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016 (pp. 1390-1398). [7789665] IEEE Computer Society. https://doi.org/10.1109/CVPRW.2016.175

Four Dimensional Image Registration for Intravital Microscopy. / Fu, Chichen; Gadgil, Neeraj; Tahboub, Khalid K.; Salama, Paul; Dunn, Kenneth; Delp, Edward J.

Proceedings - 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016. IEEE Computer Society, 2016. p. 1390-1398 7789665.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fu, C, Gadgil, N, Tahboub, KK, Salama, P, Dunn, K & Delp, EJ 2016, Four Dimensional Image Registration for Intravital Microscopy. in Proceedings - 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016., 7789665, IEEE Computer Society, pp. 1390-1398, 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016, Las Vegas, United States, 6/26/16. https://doi.org/10.1109/CVPRW.2016.175
Fu C, Gadgil N, Tahboub KK, Salama P, Dunn K, Delp EJ. Four Dimensional Image Registration for Intravital Microscopy. In Proceedings - 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016. IEEE Computer Society. 2016. p. 1390-1398. 7789665 https://doi.org/10.1109/CVPRW.2016.175
Fu, Chichen ; Gadgil, Neeraj ; Tahboub, Khalid K. ; Salama, Paul ; Dunn, Kenneth ; Delp, Edward J. / Four Dimensional Image Registration for Intravital Microscopy. Proceedings - 29th IEEE Conference on Computer Vision and Pattern Recognition Workshops, CVPRW 2016. IEEE Computer Society, 2016. pp. 1390-1398
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