Four-vessel occlusion model using aged male Wistar rats: a reliable model to resolve the discrepancy related to age in cerebral ischemia research

Jesús Ancer-Rodríguez, Eliud Enrique Villarreal-Silva, Rodolfo Amador Salazar-Ybarra, Oscar Quiroga-García, Humberto Rodríguez-Rocha, Aracely García-García, Rodolfo Morales-Avalos, Jesús Alberto Morales-Gómez, Alejandro Quiroga-Garza, Odila Saucedo-Cárdenas, Zao Cheng Xu, Rodrigo Enrique Elizondo-Omaña, Angel Raymundo Martínez-Ponce-de-León, Santos Guzmán-López

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Abstract

Animal models of cerebral ischemia have typically been established and performed using young animals, even though cerebral ischemia (CI) affects primarily elderly patients. This situation represents a discrepancy that complicates the translation of novel therapeutic strategies for CI. Models of transient global CI using aged animals have demonstrated an apparent neuroprotective effect on CA1 hippocampal neurons; however, this effect is not completely understood. Our study used a model in which young (3–6 months) and aged (18–21 months) male Wistar rats were subjected to 15 min of transient global CI using the four-vessel occlusion (4 VO) model. We determined that the 4 VO model can be performed on aged rats with a slight increase in mortality rate. In aged rats, the morphological damage was completely established by the 4th day after reperfusion, displaying no difference from their younger counterparts. These results demonstrated the lack of a neuroprotective effect of aging on CA1 hippocampal neurons in aged male Wistar rats. This study determined and characterized the morphological damage to the CA1 area after 15 min of 4 VO in aged male Wistar rats, validating the use of this model in CI and aging research.

Original languageEnglish (US)
Pages (from-to)226-237
Number of pages12
JournalAnatomical Science International
Volume91
Issue number3
DOIs
StatePublished - Jun 1 2016

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Keywords

  • Aging
  • Animal models
  • Brain ischemia
  • Global cerebral ischemia
  • Hippocampal CA1 region

ASJC Scopus subject areas

  • Anatomy

Cite this

Ancer-Rodríguez, J., Villarreal-Silva, E. E., Salazar-Ybarra, R. A., Quiroga-García, O., Rodríguez-Rocha, H., García-García, A., Morales-Avalos, R., Morales-Gómez, J. A., Quiroga-Garza, A., Saucedo-Cárdenas, O., Xu, Z. C., Elizondo-Omaña, R. E., Martínez-Ponce-de-León, A. R., & Guzmán-López, S. (2016). Four-vessel occlusion model using aged male Wistar rats: a reliable model to resolve the discrepancy related to age in cerebral ischemia research. Anatomical Science International, 91(3), 226-237. https://doi.org/10.1007/s12565-015-0286-x