FOXP3 expression and nodal metastasis of breast cancer

Yesim Polar, Mangesh A. Thorat, Payal Sojitra, Rashmil Saxena, Sunil Badve

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose T regulatory cells, a subset of T lymphocytes, function to suppress immune responses. FOXP3, a member of the forkhead family of transcription factors, is a good marker for T regulatory cells. Since sentinel nodes are important sites of immunomodulation in breast cancer, we studied the association between T regulatory cells and nodal metastasis using FOXP3 expression in sentinel nodes with and without metastatic breast carcinoma. Methods Following sample size calculations, we selected 140 sentinel nodes from breast cancer patients; 70 with metastasis (sentinel node×) and 70 without metastasis (sentinel node-). FOXP3 expression in sentinel nodes was studied by immunohistochemistry. Cortical cells expressing FOXP3 were counted in 10 high power fields. Results In the evaluable cases, the node positive (n =66) and negative (n =69) groups were well balanced for all clinicopathological parameters except histological type. The mean number of T regulatory cells expressing FOXP3 (per 10 hpf) was 139 in the node positive and 132 in the node negative group (P =0.540). The mean number of T regulatory cells was 162 in patients =35 years of age (n =8) compared to 133 in older patients (P=0.250). Primary tumor pathological characteristics like tumor type, grade, size, and ER, PR, and HER2 status did not correlate with FOXP3 expression. Conclusions The number of FOXP3-expressing T regulatory cells does not differ significantly between sentinel node× and sentinel node- samples. It was also not affected by primary tumor characteristics like tumor type, grade, size, hormone receptor, and HER2 status.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalCellular Oncology
Volume36
Issue number5
DOIs
StatePublished - Oct 2013

Fingerprint

Regulatory T-Lymphocytes
Breast Neoplasms
Neoplasm Metastasis
Neoplasms
Forkhead Transcription Factors
Immunomodulation
cyhalothrin
T-Lymphocyte Subsets
Sample Size
Immunohistochemistry
Hormones

Keywords

  • Breast cancer
  • FOXP3
  • Immune response
  • Sentinel node
  • Th1
  • Th2
  • Treg
  • Tregulatorycells

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Oncology

Cite this

FOXP3 expression and nodal metastasis of breast cancer. / Polar, Yesim; Thorat, Mangesh A.; Sojitra, Payal; Saxena, Rashmil; Badve, Sunil.

In: Cellular Oncology, Vol. 36, No. 5, 10.2013, p. 405-409.

Research output: Contribution to journalArticle

Polar, Yesim ; Thorat, Mangesh A. ; Sojitra, Payal ; Saxena, Rashmil ; Badve, Sunil. / FOXP3 expression and nodal metastasis of breast cancer. In: Cellular Oncology. 2013 ; Vol. 36, No. 5. pp. 405-409.
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N2 - Purpose T regulatory cells, a subset of T lymphocytes, function to suppress immune responses. FOXP3, a member of the forkhead family of transcription factors, is a good marker for T regulatory cells. Since sentinel nodes are important sites of immunomodulation in breast cancer, we studied the association between T regulatory cells and nodal metastasis using FOXP3 expression in sentinel nodes with and without metastatic breast carcinoma. Methods Following sample size calculations, we selected 140 sentinel nodes from breast cancer patients; 70 with metastasis (sentinel node×) and 70 without metastasis (sentinel node-). FOXP3 expression in sentinel nodes was studied by immunohistochemistry. Cortical cells expressing FOXP3 were counted in 10 high power fields. Results In the evaluable cases, the node positive (n =66) and negative (n =69) groups were well balanced for all clinicopathological parameters except histological type. The mean number of T regulatory cells expressing FOXP3 (per 10 hpf) was 139 in the node positive and 132 in the node negative group (P =0.540). The mean number of T regulatory cells was 162 in patients =35 years of age (n =8) compared to 133 in older patients (P=0.250). Primary tumor pathological characteristics like tumor type, grade, size, and ER, PR, and HER2 status did not correlate with FOXP3 expression. Conclusions The number of FOXP3-expressing T regulatory cells does not differ significantly between sentinel node× and sentinel node- samples. It was also not affected by primary tumor characteristics like tumor type, grade, size, hormone receptor, and HER2 status.

AB - Purpose T regulatory cells, a subset of T lymphocytes, function to suppress immune responses. FOXP3, a member of the forkhead family of transcription factors, is a good marker for T regulatory cells. Since sentinel nodes are important sites of immunomodulation in breast cancer, we studied the association between T regulatory cells and nodal metastasis using FOXP3 expression in sentinel nodes with and without metastatic breast carcinoma. Methods Following sample size calculations, we selected 140 sentinel nodes from breast cancer patients; 70 with metastasis (sentinel node×) and 70 without metastasis (sentinel node-). FOXP3 expression in sentinel nodes was studied by immunohistochemistry. Cortical cells expressing FOXP3 were counted in 10 high power fields. Results In the evaluable cases, the node positive (n =66) and negative (n =69) groups were well balanced for all clinicopathological parameters except histological type. The mean number of T regulatory cells expressing FOXP3 (per 10 hpf) was 139 in the node positive and 132 in the node negative group (P =0.540). The mean number of T regulatory cells was 162 in patients =35 years of age (n =8) compared to 133 in older patients (P=0.250). Primary tumor pathological characteristics like tumor type, grade, size, and ER, PR, and HER2 status did not correlate with FOXP3 expression. Conclusions The number of FOXP3-expressing T regulatory cells does not differ significantly between sentinel node× and sentinel node- samples. It was also not affected by primary tumor characteristics like tumor type, grade, size, hormone receptor, and HER2 status.

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