FOXP3 interacts with hnRNPF to modulate pre-mRNA alternative splicing

Jianguang Du, Qun Wang, Steven F. Ziegler, Baohua Zhou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

FOXP3 promotes the development and function of regulatory T cells mainly through regulating the transcription of target genes. RNA alternative splicing has been implicated in a wide range of physiological and pathophysiological processes. We report here that FOXP3 associates with heterogeneous nuclear ribonucleoprotein (hnRNP) F through the exon 2-encoded region of FOXP3 and the second quasi-RNA recognition motif (qRRM) of hnRNPF. FOXP3 represses the ability of hnRNPF to bind to its target pre-mRNA and thus modulates RNA alternative splicing. Furthermore, overexpression of mouse hnRNPF in in vitro-differentiated regulatory T cells (Tregs) reduced their suppressive function. Thus, our studies identify a novel mechanism by which FOXP3 regulates mRNA alternative splicing to modulate the function of regulatory T cells.

Original languageEnglish (US)
Pages (from-to)10235-10244
Number of pages10
JournalJournal of Biological Chemistry
Volume293
Issue number26
DOIs
StatePublished - Jan 1 2018

Fingerprint

T-cells
RNA Precursors
Alternative Splicing
Regulatory T-Lymphocytes
Heterogeneous-Nuclear Ribonucleoproteins
Physiological Phenomena
Transcription
Exons
Genes
RNA
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

FOXP3 interacts with hnRNPF to modulate pre-mRNA alternative splicing. / Du, Jianguang; Wang, Qun; Ziegler, Steven F.; Zhou, Baohua.

In: Journal of Biological Chemistry, Vol. 293, No. 26, 01.01.2018, p. 10235-10244.

Research output: Contribution to journalArticle

Du, Jianguang ; Wang, Qun ; Ziegler, Steven F. ; Zhou, Baohua. / FOXP3 interacts with hnRNPF to modulate pre-mRNA alternative splicing. In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 26. pp. 10235-10244.
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