Frequent Loss of Expression and Loss of Heterozygosity of the Putative Tumor Suppressor Gene DCC in Prostatic Carcinomas

David Grignon, Wael Sakr, Yong Q. Chen

Research output: Contribution to journalArticle

145 Scopus citations

Abstract

The putative tumor suppressor gene DCC has been shown to be frequently lost or expressed at low levels in colorectal, gastric, pancreatic, and esophageal carcinomas. In the present study, the DCC gene and its mRNA expression in human and rat prostatic carcinoma cells as well as in prostatic carcinoma tissues were examined by reverse transcriptase-polymerase chain reaction and polymerase chain reaction-loss of heterozygosity. The DCC gene was present and expressed in normal prostatic cells. However, its expression was decreased or undetectable in all prostatic carcinoma cells from either humans (4 cell lines) or rats (5 cell lines). In patients, 12 of 14 cases (86%) showed reduced DCC expression and 5 of 11 informative cases (45%) showed loss of heterozygosity at the DCC locus. These results demonstrate that loss of DCC expression and loss of heterozygosity at the DCC locus are a frequent feature of prostatic carcinoma cells.

Original languageEnglish (US)
Pages (from-to)2723-2727
Number of pages5
JournalCancer Research
Volume53
Issue number12
StatePublished - Jun 1993

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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