From tissue phenotype to proteotype: Sensitive protein identification in microdissected tumor tissue

Zhengping Zhuang, Steve Huang, Jeff A. Kowalak, Ying Shi, Jingqi Lei, Makoto Furuta, Youn Soo Lee, Irina A. Lubensky, Griffin P. Rodgers, Albert S. Cornelius, Robert J. Weil, Bin T. Teh, Alexander Vortmeyer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Correlation of disease phenotype with protein profile (proteotype) is a significant challenge for biomedical research. The main obstacles have been the need to insure sufficient quantities of pure protein sample, the reproducibility of protein display, and rapid and accurate protein identification. We present a modified approach that combines enhanced detection sensitivity with tissue microdissection from frozen primary renal cancer tissues of different histological subtypes, followed by 2D gel analysis and protein identification with MALDI mass spectrometry. We obtained reliable and highly consistent results in phenotypically similar tumors of each individual subtype by performing strict morphological control of the analyzed tumor cells without physical or chemical alteration of the frozen tissue samples. By application of non-oxidizing silver staining, proteins were resolved and identified with high levels of specificity and sensitivity. This new combination of techniques allows not only for sensitive identification of specific protein patterns that correspond to a histological tumor phenotype, but also for identification of specific disease-associated protein targets.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalInternational Journal of Oncology
Volume28
Issue number1
StatePublished - Jan 1 2006
Externally publishedYes

Fingerprint

Phenotype
Neoplasms
Proteins
Silver Proteins
Microdissection
Silver Staining
Kidney Neoplasms
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Biomedical Research
Mass Spectrometry
Gels
Sensitivity and Specificity

Keywords

  • Profiling
  • Proteomics
  • RCC

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

From tissue phenotype to proteotype : Sensitive protein identification in microdissected tumor tissue. / Zhuang, Zhengping; Huang, Steve; Kowalak, Jeff A.; Shi, Ying; Lei, Jingqi; Furuta, Makoto; Lee, Youn Soo; Lubensky, Irina A.; Rodgers, Griffin P.; Cornelius, Albert S.; Weil, Robert J.; Teh, Bin T.; Vortmeyer, Alexander.

In: International Journal of Oncology, Vol. 28, No. 1, 01.01.2006, p. 103-110.

Research output: Contribution to journalArticle

Zhuang, Z, Huang, S, Kowalak, JA, Shi, Y, Lei, J, Furuta, M, Lee, YS, Lubensky, IA, Rodgers, GP, Cornelius, AS, Weil, RJ, Teh, BT & Vortmeyer, A 2006, 'From tissue phenotype to proteotype: Sensitive protein identification in microdissected tumor tissue', International Journal of Oncology, vol. 28, no. 1, pp. 103-110.
Zhuang Z, Huang S, Kowalak JA, Shi Y, Lei J, Furuta M et al. From tissue phenotype to proteotype: Sensitive protein identification in microdissected tumor tissue. International Journal of Oncology. 2006 Jan 1;28(1):103-110.
Zhuang, Zhengping ; Huang, Steve ; Kowalak, Jeff A. ; Shi, Ying ; Lei, Jingqi ; Furuta, Makoto ; Lee, Youn Soo ; Lubensky, Irina A. ; Rodgers, Griffin P. ; Cornelius, Albert S. ; Weil, Robert J. ; Teh, Bin T. ; Vortmeyer, Alexander. / From tissue phenotype to proteotype : Sensitive protein identification in microdissected tumor tissue. In: International Journal of Oncology. 2006 ; Vol. 28, No. 1. pp. 103-110.
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