Function and structure in the diphenylamine exposed kidney

K. D. Gardner, S. Solomon, W. W. Fitzgerrel, Andrew Evan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Standard micropuncture and microdissection techniques were used to examine the function and structure of nephrons in rats whose kidneys were made cystic by dietary exposure to diphenylamine. Heterogeneity characterized the lesion, with dilation and frank cyst formation occurring in 5-30% of nephrons. Elevated intraluminal hydrostatic pressures, occurring in the absence of increased glomerular filtration or decreased net water reabsorption, were recorded in dilated, but not in nondilated nephrons. Structural studies demonstrated communication of dilated nephrons with cysts, concretions of debris within tubular lumens, evidence of extrinsic pressure by cysts on adjacent tubules, and apparent luminal narrowing of some proximal tubules. These observations were used to explain prolonged loop of Henle transit times and occasional failure to detect [3H]inulin excretion after microperfusion into dilated tubules. It was concluded that the elevated hydrostatic pressures in the dilated nephrons of diphenylamine exposed kidneys were the consequence of variably severe and frequently incomplete tubular occlusion. These findings support the hypothesis that cyst formation is a consequence of partial obstruction and elevated intratubular pressure in this model and perhaps in other susceptible mammalian kidneys.

Original languageEnglish (US)
Pages (from-to)796-806
Number of pages11
JournalJournal of Clinical Investigation
Volume57
Issue number3
StatePublished - 1976
Externally publishedYes

Fingerprint

Diphenylamine
Nephrons
Cysts
Kidney
Hydrostatic Pressure
Loop of Henle
Pressure
Microdissection
Inulin
Punctures
Dilatation
Communication
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Gardner, K. D., Solomon, S., Fitzgerrel, W. W., & Evan, A. (1976). Function and structure in the diphenylamine exposed kidney. Journal of Clinical Investigation, 57(3), 796-806.

Function and structure in the diphenylamine exposed kidney. / Gardner, K. D.; Solomon, S.; Fitzgerrel, W. W.; Evan, Andrew.

In: Journal of Clinical Investigation, Vol. 57, No. 3, 1976, p. 796-806.

Research output: Contribution to journalArticle

Gardner, KD, Solomon, S, Fitzgerrel, WW & Evan, A 1976, 'Function and structure in the diphenylamine exposed kidney', Journal of Clinical Investigation, vol. 57, no. 3, pp. 796-806.
Gardner, K. D. ; Solomon, S. ; Fitzgerrel, W. W. ; Evan, Andrew. / Function and structure in the diphenylamine exposed kidney. In: Journal of Clinical Investigation. 1976 ; Vol. 57, No. 3. pp. 796-806.
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