Functional analysis of the domains of the C. elegans Ror receptor tyrosine kinase CAM-1

Changsung Kim, Wayne C. Forrester

Research output: Contribution to journalArticle

49 Scopus citations


cam-1 encodes a Caenorhabditis elegans orphan receptor tyrosine kinase (RTK) of the Ror family that is required for cell migration and to orient cell polarity. Ror RTKs share a common domain structure. The predicted extracellular region contains immunoglobulin (Ig), cysteine-rich (CRD), and kringle (Kri) domains. Intracellularly are tyrosine kinase (Kin) and serine- and threonine (S/T)-rich domains. To investigate the functional requirement for CAM-1 domains in mediating cell migration, we engineered deletions that remove various domains and assessed the ability of these CAM-1 derivatives to rescue cam-1 mutant phenotypes. We find that the Ig, Kri, Kin, and S/T domains are dispensable for cell migration, but the CRD is required. Surprisingly, the entire intracellular region of CAM-1 is not required for proper cell migration. Most notably, a version of CAM-1 from which all domains besides the CRD and transmembrane domains have been deleted is able to rescue the migration of a single cell type, although not those of other cell types. Our results show that CAM-1 does not function exclusively as a canonical RTK and that it may function, at least in part, to regulate the distribution of a secreted ligand - possibly a Wnt protein.

Original languageEnglish (US)
Pages (from-to)376-390
Number of pages15
JournalDevelopmental Biology
Issue number2
StatePublished - Dec 15 2003


  • cam-1
  • Cell migration
  • Cell polarity
  • kin-8
  • Ror
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology

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