Functional B-1 progenitor cells are present in the hematopoietic stem cell-deficient embryo and depend on Cbfβ for their development

Michihiro Kobayashi, W. Christopher Shelley, Wooseok Seo, Sasidhar Vemula, Yang Lin, Yan Liu, Reuben Kapur, Ichiro Taniuchi, Momoko Yoshimoto

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The fetal liver is a major hematopoietic site containing progenitor cells that give rise to nearly all blood cells, including B-1 cells. Because the fetal liver is not a de novo site of hematopoietic stem cell (HSC) or progenitor-cell emergence, it must be seeded by yolk sac (YS)-derived erythromyeloid progenitors at embryonic day (E) 8.5-E10 and aorta-gonado-mesonephros (AGM)-derived HSCs at E10.5-E11.5. Although the B-1 progenitor cell pool in the fetal liver is considered to be of HSC origin, we have previously proposed that YS-derived B-1 progenitors may also contribute to this pool. Until now, it has been impossible to determine whether HSC-independent B-1 progenitor cells exist in the fetal liver. Here, we demonstrate the presence of transplantable fetal-liver B-1 and marginal zone B progenitor cells in genetically engineered HSC-deficient embryos. HSC-deficient YS and AGM tissues produce B-1 progenitors in vitro and thus may serve as sites of origin for the B-1 progenitors that seed the fetal liver. Furthermore, we have found that core-binding factor beta (Cbfβ) expression is required for fetal-liver B-1 progenitor cell maturation and expansion. Our data provide, to our knowledge, the first evidence for the presence of B-1 progenitor cells in the fetal liver that arise independently of HSCs and implicate Cbfβ as a critical molecule in the development of this lineage.

Original languageEnglish
Pages (from-to)12151-12156
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number33
DOIs
StatePublished - Aug 19 2014

Fingerprint

Core Binding Factor beta Subunit
B-Lymphoid Precursor Cells
Hematopoietic Stem Cells
Embryonic Structures
Liver
Yolk Sac
Mesonephros
Aorta
Stem Cells
Blood Cells
Seeds

Keywords

  • Hemogenic endothelial cell
  • Immune layered model
  • TEK

ASJC Scopus subject areas

  • General

Cite this

Functional B-1 progenitor cells are present in the hematopoietic stem cell-deficient embryo and depend on Cbfβ for their development. / Kobayashi, Michihiro; Shelley, W. Christopher; Seo, Wooseok; Vemula, Sasidhar; Lin, Yang; Liu, Yan; Kapur, Reuben; Taniuchi, Ichiro; Yoshimoto, Momoko.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 33, 19.08.2014, p. 12151-12156.

Research output: Contribution to journalArticle

Kobayashi, Michihiro ; Shelley, W. Christopher ; Seo, Wooseok ; Vemula, Sasidhar ; Lin, Yang ; Liu, Yan ; Kapur, Reuben ; Taniuchi, Ichiro ; Yoshimoto, Momoko. / Functional B-1 progenitor cells are present in the hematopoietic stem cell-deficient embryo and depend on Cbfβ for their development. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 33. pp. 12151-12156.
@article{d07d1ff7f318448694b934be40fee313,
title = "Functional B-1 progenitor cells are present in the hematopoietic stem cell-deficient embryo and depend on Cbfβ for their development",
abstract = "The fetal liver is a major hematopoietic site containing progenitor cells that give rise to nearly all blood cells, including B-1 cells. Because the fetal liver is not a de novo site of hematopoietic stem cell (HSC) or progenitor-cell emergence, it must be seeded by yolk sac (YS)-derived erythromyeloid progenitors at embryonic day (E) 8.5-E10 and aorta-gonado-mesonephros (AGM)-derived HSCs at E10.5-E11.5. Although the B-1 progenitor cell pool in the fetal liver is considered to be of HSC origin, we have previously proposed that YS-derived B-1 progenitors may also contribute to this pool. Until now, it has been impossible to determine whether HSC-independent B-1 progenitor cells exist in the fetal liver. Here, we demonstrate the presence of transplantable fetal-liver B-1 and marginal zone B progenitor cells in genetically engineered HSC-deficient embryos. HSC-deficient YS and AGM tissues produce B-1 progenitors in vitro and thus may serve as sites of origin for the B-1 progenitors that seed the fetal liver. Furthermore, we have found that core-binding factor beta (Cbfβ) expression is required for fetal-liver B-1 progenitor cell maturation and expansion. Our data provide, to our knowledge, the first evidence for the presence of B-1 progenitor cells in the fetal liver that arise independently of HSCs and implicate Cbfβ as a critical molecule in the development of this lineage.",
keywords = "Hemogenic endothelial cell, Immune layered model, TEK",
author = "Michihiro Kobayashi and Shelley, {W. Christopher} and Wooseok Seo and Sasidhar Vemula and Yang Lin and Yan Liu and Reuben Kapur and Ichiro Taniuchi and Momoko Yoshimoto",
year = "2014",
month = "8",
day = "19",
doi = "10.1073/pnas.1407370111",
language = "English",
volume = "111",
pages = "12151--12156",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "33",

}

TY - JOUR

T1 - Functional B-1 progenitor cells are present in the hematopoietic stem cell-deficient embryo and depend on Cbfβ for their development

AU - Kobayashi, Michihiro

AU - Shelley, W. Christopher

AU - Seo, Wooseok

AU - Vemula, Sasidhar

AU - Lin, Yang

AU - Liu, Yan

AU - Kapur, Reuben

AU - Taniuchi, Ichiro

AU - Yoshimoto, Momoko

PY - 2014/8/19

Y1 - 2014/8/19

N2 - The fetal liver is a major hematopoietic site containing progenitor cells that give rise to nearly all blood cells, including B-1 cells. Because the fetal liver is not a de novo site of hematopoietic stem cell (HSC) or progenitor-cell emergence, it must be seeded by yolk sac (YS)-derived erythromyeloid progenitors at embryonic day (E) 8.5-E10 and aorta-gonado-mesonephros (AGM)-derived HSCs at E10.5-E11.5. Although the B-1 progenitor cell pool in the fetal liver is considered to be of HSC origin, we have previously proposed that YS-derived B-1 progenitors may also contribute to this pool. Until now, it has been impossible to determine whether HSC-independent B-1 progenitor cells exist in the fetal liver. Here, we demonstrate the presence of transplantable fetal-liver B-1 and marginal zone B progenitor cells in genetically engineered HSC-deficient embryos. HSC-deficient YS and AGM tissues produce B-1 progenitors in vitro and thus may serve as sites of origin for the B-1 progenitors that seed the fetal liver. Furthermore, we have found that core-binding factor beta (Cbfβ) expression is required for fetal-liver B-1 progenitor cell maturation and expansion. Our data provide, to our knowledge, the first evidence for the presence of B-1 progenitor cells in the fetal liver that arise independently of HSCs and implicate Cbfβ as a critical molecule in the development of this lineage.

AB - The fetal liver is a major hematopoietic site containing progenitor cells that give rise to nearly all blood cells, including B-1 cells. Because the fetal liver is not a de novo site of hematopoietic stem cell (HSC) or progenitor-cell emergence, it must be seeded by yolk sac (YS)-derived erythromyeloid progenitors at embryonic day (E) 8.5-E10 and aorta-gonado-mesonephros (AGM)-derived HSCs at E10.5-E11.5. Although the B-1 progenitor cell pool in the fetal liver is considered to be of HSC origin, we have previously proposed that YS-derived B-1 progenitors may also contribute to this pool. Until now, it has been impossible to determine whether HSC-independent B-1 progenitor cells exist in the fetal liver. Here, we demonstrate the presence of transplantable fetal-liver B-1 and marginal zone B progenitor cells in genetically engineered HSC-deficient embryos. HSC-deficient YS and AGM tissues produce B-1 progenitors in vitro and thus may serve as sites of origin for the B-1 progenitors that seed the fetal liver. Furthermore, we have found that core-binding factor beta (Cbfβ) expression is required for fetal-liver B-1 progenitor cell maturation and expansion. Our data provide, to our knowledge, the first evidence for the presence of B-1 progenitor cells in the fetal liver that arise independently of HSCs and implicate Cbfβ as a critical molecule in the development of this lineage.

KW - Hemogenic endothelial cell

KW - Immune layered model

KW - TEK

UR - http://www.scopus.com/inward/record.url?scp=84906309039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906309039&partnerID=8YFLogxK

U2 - 10.1073/pnas.1407370111

DO - 10.1073/pnas.1407370111

M3 - Article

C2 - 25092306

AN - SCOPUS:84906309039

VL - 111

SP - 12151

EP - 12156

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 33

ER -