Functional characterization of a panel of high-grade serous ovarian cancer cell lines as representative experimental models of the disease

James Haley, Sunil Tomar, Nicholas Pulliam, Sen Xiong, Susan Perkins, Adam R. Karpf, Sumegha Mitra, Kenneth Nephew, Anirban Mitra

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Genomic analysis of ovarian cancer cell lines has revealed a panel that best represents the most common ovarian cancer subtype, high-grade serous ovarian cancer (HGSOC). However, these HGSOC-like cell lines have not been extensively applied by ovarian cancer researchers to date, and the most commonly used cell lines in the ovarian cancer field do not genetically resemble the major clinical type of the disease. For the HGSOC-like lines to serve as suitable models, they need to be characterized for common functional assays. To achieve that objective, we systematically studied a panel of HGSOC cells CAOV3, COV362, Kuramochi, OVCAR4, OVCAR5, OVCAR8, OVSAHO and SNU119 for migration, invasion, proliferation, clonogenicity, EMT phenotype and cisplatin resistance. They exhibited a range of efficacies and OVCAR5, OVCAR8 and Kuramochi were the most aggressive. SNU119 and OVSAHO cells demonstrated the lowest functional activities. Wide differences in expression of EMT markers were observed between cell lines. SNU119 were the most epithelial and OVCAR8 had the most mesenchymal phenotype. COV362 was the most resistant to cisplatin while CAOV3 was the most sensitive. Taken together, our systematic characterization represents a valuable resource to help guide the application of HGSOC cells by the cancer research community.

Original languageEnglish (US)
Pages (from-to)32810-32820
Number of pages11
JournalOncotarget
Volume7
Issue number22
DOIs
StatePublished - May 31 2016

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Ovarian Neoplasms
Theoretical Models
Cell Line
Cisplatin
Phenotype
Research Personnel
Research

Keywords

  • Clonogenicity
  • Invasion
  • Migration
  • Ovarian cancer
  • Proliferation

ASJC Scopus subject areas

  • Oncology

Cite this

Functional characterization of a panel of high-grade serous ovarian cancer cell lines as representative experimental models of the disease. / Haley, James; Tomar, Sunil; Pulliam, Nicholas; Xiong, Sen; Perkins, Susan; Karpf, Adam R.; Mitra, Sumegha; Nephew, Kenneth; Mitra, Anirban.

In: Oncotarget, Vol. 7, No. 22, 31.05.2016, p. 32810-32820.

Research output: Contribution to journalArticle

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