Functional effects of overexpressing junctin in mouse hearts

J. Neumann, P. Boknik, Larry Jones

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Junctin (JUN) is a protein localized to the junctional sarcoplasmic reticulum (SR) that binds to calsequestrin which is a high capacity Ca2+ binding protein of the SR. In order to understand the functional role of JUN, transgenic mice (TG) were generated overexpressing dog JUN under the control of the alpha myosin heavy chain promoter. JUN was measured by quantitative immunoblotting with an antibody specific to dog JUN. No dog JUN was detectable in control mouse hearts (Ctr), while in (TG) the level of JUN (in arbitrary units) amounted to 1.24 ± 0.09, 1.12 ± 0.13, 0.92 ± 0.10, 0.71 ± 0.07 in left atria, right atria, left ventricles and right ventricles, resp. (n = 7). Heart weights were unaltered (TG 116 ± 3.6 mg vs. Ctr: 106 ± 5.1 mg). No gross anatomical abnormalities were seen. Time of relaxation (left atria, 1 Hz) in the presence of 5.4 mM Ca2+ was prolonged in TG (55 ± 4 ms) vs. Ctr (45 ± 2.7 ms, n = 6-8). Thus, JUN may play an important role in the Ca2+ handling by the SR.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Transgenic Mice
Sarcoplasmic Reticulum
Heart Atria
Calsequestrin
heart
sarcoplasmic reticulum
Ventricular Myosins
Myosin Heavy Chains
genetically modified organisms
mice
Dogs
Heart Ventricles
Carrier Proteins
calcium
dogs
Antibodies
Immunoblotting
myosin heavy chains
myosin
immunoblotting

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Functional effects of overexpressing junctin in mouse hearts. / Neumann, J.; Boknik, P.; Jones, Larry.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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