Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast

Sabire Ozcan, Amber L. Mosley, Bishwa K. Aryal

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The pancreas-specific transcription factor Pdx-1 is important for pancreas development and β-cell specific gene expression in insulin-producing cells. We have expressed the mouse PDX-1 gene in the yeast Saccharomyces cerevisiae and characterized its functional domains. Pdx-1 functions as a strong activator in yeast and stimulates gene expression by more than 80-fold. The transcriptional activation domain of Pdx-1 is located within the first 144 amino-terminal amino acids. Pdx-1 is also able to bind and activate transcription from the A3 element of the human insulin gene promoter in yeast. Analysis of the effects of two-point mutations (Q59L and R197H) in the PDX-1 gene found in type II diabetes patients showed that both point mutations interfere with the ability of Pdx-1 to bind to DNA and to activate transcription in yeast.

Original languageEnglish (US)
Pages (from-to)724-729
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume295
Issue number3
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Keywords

  • Activator
  • Gene expression
  • Pancreas
  • Pdx-1
  • Transcription factor
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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