Functional microRNA involved in endometriosis

Shannon Hawkins, Chad J. Creighton, Derek Y. Han, Azam Zariff, Matthew L. Anderson, Preethi H. Gunaratne, Martin M. Matzuk

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Endometriosis is a common disease seen by gynecologists. Clinical features involve pelvic pain and unexplained infertility. Although endometriosis is pathologically characterized by endometrial tissue outside the normal uterine location, endometriosis is otherwise not easily explained. Endometriomas, endometriotic cysts of the ovary, typically cause pain and distortion of pelvic anatomy. To begin to understand the pathogenesis of endometriomas, we describe the first transcriptome-microRNAome analysis of endometriomas and eutopic endometrium using next-generation sequencing technology. Using this approach, we generated a total of more than 54 million independent small RNA reads from our 19 clinical samples. At the microRNA level, we found 10micro RNA that were up-regulated (miR-202, 193a-3p, 29c, 708, 509-3-5p, 574-3p, 193a-5p, 485-3p, 100, and 720) and 12 micro RNA that were down-regulated (miR-504, 141, 429,203,10a, 200b, 873, 200c, 200a, 449b, 375, and 34c-5p) in endometriomas compared with endometrium. Using in silico prediction algorithms, we correlated these micro RNA with their corresponding differentially expressed mRNA targets. To validate the functional roles of micro RNA, we manipulated levels of miR-29c in an in vitro system of primary cultures of human endometrial stromal fibroblasts. Extracellular matrix genes that were potential targets of miR-29c in silico were significantly down-regulated using this biological in vitro system. In vitro functional studies using luciferase reporter constructs further confirmed that miR-29c directly affects specific extracellular matrix genes that are dysregulated in endometriomas. Thus, miR-29c and other abnormally regulated micro RNA appear to play important roles in the pathophysiology of uterine function and dysfunction. (Molecular Endocrinology 25: 821-832, 2011).

Original languageEnglish (US)
Pages (from-to)821-832
Number of pages12
JournalMolecular Endocrinology
Volume25
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

Fingerprint

Endometriosis
MicroRNAs
Pelvic Pain
Endometrium
Computer Simulation
Extracellular Matrix
RNA
Endocrinology
Gene Expression Profiling
Luciferases
Infertility
Genes
Cysts
Ovary
Anatomy
Fibroblasts
Technology
Messenger RNA
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Hawkins, S., Creighton, C. J., Han, D. Y., Zariff, A., Anderson, M. L., Gunaratne, P. H., & Matzuk, M. M. (2011). Functional microRNA involved in endometriosis. Molecular Endocrinology, 25(5), 821-832. https://doi.org/10.1210/me.2010-0371

Functional microRNA involved in endometriosis. / Hawkins, Shannon; Creighton, Chad J.; Han, Derek Y.; Zariff, Azam; Anderson, Matthew L.; Gunaratne, Preethi H.; Matzuk, Martin M.

In: Molecular Endocrinology, Vol. 25, No. 5, 05.2011, p. 821-832.

Research output: Contribution to journalArticle

Hawkins, S, Creighton, CJ, Han, DY, Zariff, A, Anderson, ML, Gunaratne, PH & Matzuk, MM 2011, 'Functional microRNA involved in endometriosis', Molecular Endocrinology, vol. 25, no. 5, pp. 821-832. https://doi.org/10.1210/me.2010-0371
Hawkins S, Creighton CJ, Han DY, Zariff A, Anderson ML, Gunaratne PH et al. Functional microRNA involved in endometriosis. Molecular Endocrinology. 2011 May;25(5):821-832. https://doi.org/10.1210/me.2010-0371
Hawkins, Shannon ; Creighton, Chad J. ; Han, Derek Y. ; Zariff, Azam ; Anderson, Matthew L. ; Gunaratne, Preethi H. ; Matzuk, Martin M. / Functional microRNA involved in endometriosis. In: Molecular Endocrinology. 2011 ; Vol. 25, No. 5. pp. 821-832.
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