Functional role of BK virus tumor antigens in transformation

Harikrishna Nakshatri, M. M. Pater, A. Pater

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have examined the role of the human papovavirus BK virus (BKV) tumor (T) antigen(s) in the maintenance of transformation and have identified the domain of T antigen essential for transformation. BKV-transformed BHK 21 and NIH 3T3 cells expressing antisense T-antigen RNA lose their ability to grow in soft agar, indicating the need for continued expression of T antigen for the maintenance of the transformed phenotype. Experiments using translation termination linker insertion and deletion mutagenesis of BKV T antigen demonstrate that amino acids 356 to 384 are essential for transformation. Although BKV T antigen shares 100, 95, and 82% amino acid homology with that of simian virus 40 (SV40) for the nuclear localization signal, p53-binding domain, and DNA-binding domain, respectively, the transformation domains of BKV and SV40 T antigens share only 54% homology. ALso, BKV T antigen lacks a substantial portion of the ATPase domain of SV40, and our results indicate that dispensability of the remaining portion for transformation by this protein. We suggest that the differences in the amino acids in the identified transformation domains together with the differences in the ATPase domains may account for the differences in the transformation potentials of the two proteins.

Original languageEnglish (US)
Pages (from-to)4613-4621
Number of pages9
JournalJournal of Virology
Volume62
Issue number12
StatePublished - 1988
Externally publishedYes

Fingerprint

BK Virus
Viral Tumor Antigens
Neoplasm Antigens
antigens
viruses
neoplasms
Simian virus 40
Amino Acids
amino acids
adenosinetriphosphatase
Adenosine Triphosphatases
Maintenance
Nuclear Localization Signals
nuclear localization signals
NIH 3T3 Cells
DNA-binding domains
Insertional Mutagenesis
mutagenesis
translation (genetics)
Agar

ASJC Scopus subject areas

  • Immunology

Cite this

Functional role of BK virus tumor antigens in transformation. / Nakshatri, Harikrishna; Pater, M. M.; Pater, A.

In: Journal of Virology, Vol. 62, No. 12, 1988, p. 4613-4621.

Research output: Contribution to journalArticle

Nakshatri, H, Pater, MM & Pater, A 1988, 'Functional role of BK virus tumor antigens in transformation', Journal of Virology, vol. 62, no. 12, pp. 4613-4621.
Nakshatri, Harikrishna ; Pater, M. M. ; Pater, A. / Functional role of BK virus tumor antigens in transformation. In: Journal of Virology. 1988 ; Vol. 62, No. 12. pp. 4613-4621.
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