Functional screening of intracardiac cell transplants using two-photon fluorescence microscopy

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Although the adult mammalian myocardium exhibits a limited ability to undergo regenerative growth, its intrinsic renewal rate is insufficient to compensate for myocyte loss during cardiac disease. Transplantation of donor cardiomyocytes or cardiomyogenic stemcells is considered a promising strategy for reconstitution of cardiac mass, provided the engrafted cells functionally integrate with host myocardium and actively contribute to its contractile force. The authors previously developed a two-photon fluorescence microscopy-based assay that allows in situ screening of donor cell function after intracardiac delivery of the cells. This report reviews the techniques of two-photon fluorescence microscopy and summarizes its application for quantifying the extent to which a variety of donor cell types stably and functionally couple with the recipient myocardium.

Original languageEnglish (US)
Pages (from-to)929-937
Number of pages9
JournalPediatric Cardiology
Volume33
Issue number6
DOIs
StatePublished - Aug 2012

Keywords

  • Cellular transplantation
  • Intracellular calcium regulation
  • Myocardial regeneration
  • Two-photon fluorescence microscopy

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pediatrics, Perinatology, and Child Health

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