Functional studies in living animals using multiphoton microscopy

Research output: Contribution to journalArticle

Abstract

In vivo microscopy is a powerful method for studying fundamental issues of physiology and pathophysiology. The recent development of multiphoton fluorescence microscopy has extended the reach of in vivo microscopy, supporting high-resolution imaging deep into the tissues and organs of living animals. As compared with other in vivo imaging techniques, multiphoton microscopy is uniquely capable of providing a window into cellular and subcellular processes in the context of the intact, functioning animal. In addition, the ability to collect multiple colors of fluorescence from the same sample makes in vivo microscopy uniquely capable of characterizing up to three parameters from the same volume, supporting powerful correlative analyses. Since its invention in 1990, multiphoton microscopy has been increasingly applied to numerous areas of medical investigation, providing invaluable insights into cell physiology and pathology. However, researchers have only begun to realize the true potential of this powerful technology as it has proliferated beyond the laboratories of a relatively few pioneers. In this article we present an overview of the advantages and limitations of multiphoton microscopy as applied to in vivo imaging. We also review specific examples of the application of in vivo multiphoton microscopy to studies of physiology and pathology in a variety of organs including the brain, skin, skeletal muscle, tumors, immune cells, and visceral organs.

Original languageEnglish
Pages (from-to)66-77
Number of pages12
JournalILAR Journal
Volume53
Issue number1
StatePublished - 2012

Fingerprint

Microscopy
microscopy
Physiology
Microscopic examination
Animals
Pathology
Imaging techniques
Multiphoton Fluorescence Microscopy
animals
Animal Structures
Cell Physiological Phenomena
image analysis
Fluorescence microscopy
Patents and inventions
physiology
Muscle
Tumors
Brain
Skin
Skeletal Muscle

Keywords

  • Fluorescence microscopy
  • In vivo imaging
  • In vivo microscopy
  • Multiphoton microscopy
  • Two-photon microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Animal Science and Zoology

Cite this

Functional studies in living animals using multiphoton microscopy. / Dunn, Kenneth; Sutton, Timothy.

In: ILAR Journal, Vol. 53, No. 1, 2012, p. 66-77.

Research output: Contribution to journalArticle

@article{289280f1cabb45fa85796b63e1787d28,
title = "Functional studies in living animals using multiphoton microscopy",
abstract = "In vivo microscopy is a powerful method for studying fundamental issues of physiology and pathophysiology. The recent development of multiphoton fluorescence microscopy has extended the reach of in vivo microscopy, supporting high-resolution imaging deep into the tissues and organs of living animals. As compared with other in vivo imaging techniques, multiphoton microscopy is uniquely capable of providing a window into cellular and subcellular processes in the context of the intact, functioning animal. In addition, the ability to collect multiple colors of fluorescence from the same sample makes in vivo microscopy uniquely capable of characterizing up to three parameters from the same volume, supporting powerful correlative analyses. Since its invention in 1990, multiphoton microscopy has been increasingly applied to numerous areas of medical investigation, providing invaluable insights into cell physiology and pathology. However, researchers have only begun to realize the true potential of this powerful technology as it has proliferated beyond the laboratories of a relatively few pioneers. In this article we present an overview of the advantages and limitations of multiphoton microscopy as applied to in vivo imaging. We also review specific examples of the application of in vivo multiphoton microscopy to studies of physiology and pathology in a variety of organs including the brain, skin, skeletal muscle, tumors, immune cells, and visceral organs.",
keywords = "Fluorescence microscopy, In vivo imaging, In vivo microscopy, Multiphoton microscopy, Two-photon microscopy",
author = "Kenneth Dunn and Timothy Sutton",
year = "2012",
language = "English",
volume = "53",
pages = "66--77",
journal = "ILAR Journal",
issn = "1084-2020",
publisher = "Institute for Laboratory Animal Research",
number = "1",

}

TY - JOUR

T1 - Functional studies in living animals using multiphoton microscopy

AU - Dunn, Kenneth

AU - Sutton, Timothy

PY - 2012

Y1 - 2012

N2 - In vivo microscopy is a powerful method for studying fundamental issues of physiology and pathophysiology. The recent development of multiphoton fluorescence microscopy has extended the reach of in vivo microscopy, supporting high-resolution imaging deep into the tissues and organs of living animals. As compared with other in vivo imaging techniques, multiphoton microscopy is uniquely capable of providing a window into cellular and subcellular processes in the context of the intact, functioning animal. In addition, the ability to collect multiple colors of fluorescence from the same sample makes in vivo microscopy uniquely capable of characterizing up to three parameters from the same volume, supporting powerful correlative analyses. Since its invention in 1990, multiphoton microscopy has been increasingly applied to numerous areas of medical investigation, providing invaluable insights into cell physiology and pathology. However, researchers have only begun to realize the true potential of this powerful technology as it has proliferated beyond the laboratories of a relatively few pioneers. In this article we present an overview of the advantages and limitations of multiphoton microscopy as applied to in vivo imaging. We also review specific examples of the application of in vivo multiphoton microscopy to studies of physiology and pathology in a variety of organs including the brain, skin, skeletal muscle, tumors, immune cells, and visceral organs.

AB - In vivo microscopy is a powerful method for studying fundamental issues of physiology and pathophysiology. The recent development of multiphoton fluorescence microscopy has extended the reach of in vivo microscopy, supporting high-resolution imaging deep into the tissues and organs of living animals. As compared with other in vivo imaging techniques, multiphoton microscopy is uniquely capable of providing a window into cellular and subcellular processes in the context of the intact, functioning animal. In addition, the ability to collect multiple colors of fluorescence from the same sample makes in vivo microscopy uniquely capable of characterizing up to three parameters from the same volume, supporting powerful correlative analyses. Since its invention in 1990, multiphoton microscopy has been increasingly applied to numerous areas of medical investigation, providing invaluable insights into cell physiology and pathology. However, researchers have only begun to realize the true potential of this powerful technology as it has proliferated beyond the laboratories of a relatively few pioneers. In this article we present an overview of the advantages and limitations of multiphoton microscopy as applied to in vivo imaging. We also review specific examples of the application of in vivo multiphoton microscopy to studies of physiology and pathology in a variety of organs including the brain, skin, skeletal muscle, tumors, immune cells, and visceral organs.

KW - Fluorescence microscopy

KW - In vivo imaging

KW - In vivo microscopy

KW - Multiphoton microscopy

KW - Two-photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=84865815108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865815108&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84865815108

VL - 53

SP - 66

EP - 77

JO - ILAR Journal

JF - ILAR Journal

SN - 1084-2020

IS - 1

ER -