Functional studies of the kidney of living animals using multicolor two-photon microscopy

Kenneth Dunn, Ruben M. Sandoval, Katherine Kelly, Pierre Dagher, George A. Tanner, Simon J. Atkinson, Robert Bacallao, Bruce Molitoris

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Optical microscopy, when applied to living animals, provides a powerful means of studying cell biology in the most physiologically relevant setting. The ability of two-photon microscopy to collect optical sections deep into biological tissues has opened up the field of intravital microscopy to high-resolution studies of the brain, lens, skin, and tumors. Here we present examples of the way in which two-photon microscopy can be applied to intravital studies of kidney physiology. Because the kidney is easily externalized without compromising its function, microscopy can be used to evaluate various aspects of renal function in vivo. These include cell vitality and apoptosis, fluid transport, receptor-mediated endocytosis, blood flow, and leukocyte trafficking. Efficient two-photon excitation of multiple fluorophores permits comparison of multiple probes and simultaneous characterization of multiple parameters and yields spectral information that is crucial to the interpretation of images containing uncharacterized autofluorescence. The studies described here demonstrate the way in which two-photon microscopy can provide a level of resolution previously unattainable in intravital microscopy, enabling kinetic analyses and physiological studies of the organs of living animals with subcellular resolution.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number3 52-3
StatePublished - Sep 2002

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Photons
Microscopy
Microscopic examination
Animals
Kidney
Cytology
Fluorophores
Physiology
Animal Structures
Optical microscopy
Tumors
Lenses
Brain
Skin
Endocytosis
Blood
Cell Biology
Tissue
Apoptosis
Leukocytes

Keywords

  • Fluorescence
  • In vivo
  • Multiphoton

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Functional studies of the kidney of living animals using multicolor two-photon microscopy. / Dunn, Kenneth; Sandoval, Ruben M.; Kelly, Katherine; Dagher, Pierre; Tanner, George A.; Atkinson, Simon J.; Bacallao, Robert; Molitoris, Bruce.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 3 52-3, 09.2002.

Research output: Contribution to journalArticle

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