Functional study of promoter gene polymorphisms of sclerostin

F. M. Pérez-Campo, C. Sañudo, R. Krebesova, Jesus Delgado-Calle, José A. Riancho

Research output: Contribution to journalArticle

Abstract

Sclerostin, encoded by the SOST gene, inhibits the Wnt pathway and, consequently, tends to decrease bone mass. Some polymorphisms of the SOST promoter have been associated with bone mineral density (BMD), but the molecular mechanisms involved are unknown. The aim of this study was to study the functional role of one polymorphism in vitro. We cloned the proximal promoter region of SOST gene, containing different alleles at the rs851054 SNP, in luciferase reporter vectors and transfected them into the cell lines HEK-293T, SAOS-2 and HOS-TE85. We did not find significant differences in the transcriptional activity of vectors with either the A or the G allele of the SNP. The co-transfection of vectors expressing RUNX2 and OSX markedly increased the transcriptional activity of the SOST promoter constructs (A allele, 2.5±0.9 fold, p < 0.05; G allele, 1.9±0.8 fold, p < 0.05), without significant differences between the rs851054 alleles. Moreover, no allele differences were detected in EMSAs. In conclusion, the DNA region upstream of the TSS of the SOST gene has a strong promoter activity that is enhanced by RUNX2 and OSX. Frequent allelic variants in this region have been associated with BMD, but the mechanisms involved remain to be elucidated because no functional differences between alleles were detected in vitro.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalRevista de Osteoporosis y Metabolismo Mineral
Volume8
Issue number4
StatePublished - Jan 1 2016

Keywords

  • Gene regulation
  • Polymorphisms
  • Sclerostin
  • Transfection

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Functional study of promoter gene polymorphisms of sclerostin'. Together they form a unique fingerprint.

  • Cite this

    Pérez-Campo, F. M., Sañudo, C., Krebesova, R., Delgado-Calle, J., & Riancho, J. A. (2016). Functional study of promoter gene polymorphisms of sclerostin. Revista de Osteoporosis y Metabolismo Mineral, 8(4), 121-126.