Functional study of promoter gene polymorphisms of sclerostin

F. M. Pérez-Campo, C. Sañudo, R. Krebesova, Jesus Delgado-Calle, José A. Riancho

Research output: Contribution to journalArticle

Abstract

Sclerostin, encoded by the SOST gene, inhibits the Wnt pathway and, consequently, tends to decrease bone mass. Some polymorphisms of the SOST promoter have been associated with bone mineral density (BMD), but the molecular mechanisms involved are unknown. The aim of this study was to study the functional role of one polymorphism in vitro. We cloned the proximal promoter region of SOST gene, containing different alleles at the rs851054 SNP, in luciferase reporter vectors and transfected them into the cell lines HEK-293T, SAOS-2 and HOS-TE85. We did not find significant differences in the transcriptional activity of vectors with either the A or the G allele of the SNP. The co-transfection of vectors expressing RUNX2 and OSX markedly increased the transcriptional activity of the SOST promoter constructs (A allele, 2.5±0.9 fold, p < 0.05; G allele, 1.9±0.8 fold, p < 0.05), without significant differences between the rs851054 alleles. Moreover, no allele differences were detected in EMSAs. In conclusion, the DNA region upstream of the TSS of the SOST gene has a strong promoter activity that is enhanced by RUNX2 and OSX. Frequent allelic variants in this region have been associated with BMD, but the mechanisms involved remain to be elucidated because no functional differences between alleles were detected in vitro.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalRevista de Osteoporosis y Metabolismo Mineral
Volume8
Issue number4
StatePublished - Jan 1 2016

Fingerprint

Alleles
Genes
Bone Density
Single Nucleotide Polymorphism
Wnt Signaling Pathway
Luciferases
Genetic Promoter Regions
Transfection
Bone and Bones
Cell Line
DNA
In Vitro Techniques

Keywords

  • Gene regulation
  • Polymorphisms
  • Sclerostin
  • Transfection

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Cite this

Pérez-Campo, F. M., Sañudo, C., Krebesova, R., Delgado-Calle, J., & Riancho, J. A. (2016). Functional study of promoter gene polymorphisms of sclerostin. Revista de Osteoporosis y Metabolismo Mineral, 8(4), 121-126.

Functional study of promoter gene polymorphisms of sclerostin. / Pérez-Campo, F. M.; Sañudo, C.; Krebesova, R.; Delgado-Calle, Jesus; Riancho, José A.

In: Revista de Osteoporosis y Metabolismo Mineral, Vol. 8, No. 4, 01.01.2016, p. 121-126.

Research output: Contribution to journalArticle

Pérez-Campo, FM, Sañudo, C, Krebesova, R, Delgado-Calle, J & Riancho, JA 2016, 'Functional study of promoter gene polymorphisms of sclerostin', Revista de Osteoporosis y Metabolismo Mineral, vol. 8, no. 4, pp. 121-126.
Pérez-Campo, F. M. ; Sañudo, C. ; Krebesova, R. ; Delgado-Calle, Jesus ; Riancho, José A. / Functional study of promoter gene polymorphisms of sclerostin. In: Revista de Osteoporosis y Metabolismo Mineral. 2016 ; Vol. 8, No. 4. pp. 121-126.
@article{a513c63ba5b54f21bba59bfa626e453d,
title = "Functional study of promoter gene polymorphisms of sclerostin",
abstract = "Sclerostin, encoded by the SOST gene, inhibits the Wnt pathway and, consequently, tends to decrease bone mass. Some polymorphisms of the SOST promoter have been associated with bone mineral density (BMD), but the molecular mechanisms involved are unknown. The aim of this study was to study the functional role of one polymorphism in vitro. We cloned the proximal promoter region of SOST gene, containing different alleles at the rs851054 SNP, in luciferase reporter vectors and transfected them into the cell lines HEK-293T, SAOS-2 and HOS-TE85. We did not find significant differences in the transcriptional activity of vectors with either the A or the G allele of the SNP. The co-transfection of vectors expressing RUNX2 and OSX markedly increased the transcriptional activity of the SOST promoter constructs (A allele, 2.5±0.9 fold, p < 0.05; G allele, 1.9±0.8 fold, p < 0.05), without significant differences between the rs851054 alleles. Moreover, no allele differences were detected in EMSAs. In conclusion, the DNA region upstream of the TSS of the SOST gene has a strong promoter activity that is enhanced by RUNX2 and OSX. Frequent allelic variants in this region have been associated with BMD, but the mechanisms involved remain to be elucidated because no functional differences between alleles were detected in vitro.",
keywords = "Gene regulation, Polymorphisms, Sclerostin, Transfection",
author = "P{\'e}rez-Campo, {F. M.} and C. Sa{\~n}udo and R. Krebesova and Jesus Delgado-Calle and Riancho, {Jos{\'e} A.}",
year = "2016",
month = "1",
day = "1",
language = "English (US)",
volume = "8",
pages = "121--126",
journal = "Revista de Osteoporosis y Metabolismo Mineral",
issn = "1889-836X",
publisher = "Sociedad Espanola de Investigacion Osea y del Metabolismo Mineral",
number = "4",

}

TY - JOUR

T1 - Functional study of promoter gene polymorphisms of sclerostin

AU - Pérez-Campo, F. M.

AU - Sañudo, C.

AU - Krebesova, R.

AU - Delgado-Calle, Jesus

AU - Riancho, José A.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Sclerostin, encoded by the SOST gene, inhibits the Wnt pathway and, consequently, tends to decrease bone mass. Some polymorphisms of the SOST promoter have been associated with bone mineral density (BMD), but the molecular mechanisms involved are unknown. The aim of this study was to study the functional role of one polymorphism in vitro. We cloned the proximal promoter region of SOST gene, containing different alleles at the rs851054 SNP, in luciferase reporter vectors and transfected them into the cell lines HEK-293T, SAOS-2 and HOS-TE85. We did not find significant differences in the transcriptional activity of vectors with either the A or the G allele of the SNP. The co-transfection of vectors expressing RUNX2 and OSX markedly increased the transcriptional activity of the SOST promoter constructs (A allele, 2.5±0.9 fold, p < 0.05; G allele, 1.9±0.8 fold, p < 0.05), without significant differences between the rs851054 alleles. Moreover, no allele differences were detected in EMSAs. In conclusion, the DNA region upstream of the TSS of the SOST gene has a strong promoter activity that is enhanced by RUNX2 and OSX. Frequent allelic variants in this region have been associated with BMD, but the mechanisms involved remain to be elucidated because no functional differences between alleles were detected in vitro.

AB - Sclerostin, encoded by the SOST gene, inhibits the Wnt pathway and, consequently, tends to decrease bone mass. Some polymorphisms of the SOST promoter have been associated with bone mineral density (BMD), but the molecular mechanisms involved are unknown. The aim of this study was to study the functional role of one polymorphism in vitro. We cloned the proximal promoter region of SOST gene, containing different alleles at the rs851054 SNP, in luciferase reporter vectors and transfected them into the cell lines HEK-293T, SAOS-2 and HOS-TE85. We did not find significant differences in the transcriptional activity of vectors with either the A or the G allele of the SNP. The co-transfection of vectors expressing RUNX2 and OSX markedly increased the transcriptional activity of the SOST promoter constructs (A allele, 2.5±0.9 fold, p < 0.05; G allele, 1.9±0.8 fold, p < 0.05), without significant differences between the rs851054 alleles. Moreover, no allele differences were detected in EMSAs. In conclusion, the DNA region upstream of the TSS of the SOST gene has a strong promoter activity that is enhanced by RUNX2 and OSX. Frequent allelic variants in this region have been associated with BMD, but the mechanisms involved remain to be elucidated because no functional differences between alleles were detected in vitro.

KW - Gene regulation

KW - Polymorphisms

KW - Sclerostin

KW - Transfection

UR - http://www.scopus.com/inward/record.url?scp=85046240846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046240846&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85046240846

VL - 8

SP - 121

EP - 126

JO - Revista de Osteoporosis y Metabolismo Mineral

JF - Revista de Osteoporosis y Metabolismo Mineral

SN - 1889-836X

IS - 4

ER -