Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA

M. M. Halloran, M. A. Emanuele, N. V. Emanuele, J. J. Tentler, M. R. Kelley

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We have previously reported a decrease in Luteinizing Hormone (LH) levels in serum after in vivo acute ethanol exposure in male rats. Accompanying these changes, a rapid and marked decrease of β-LH mRNA was observed. A similar decrease was not detected in the common α-subunit or β-FSH mRNA. The studies presented here examined the possible mechanisms of decreasing β-LH mRNA by using S1 nuclease protection assay to evaluate the effect of acute ethanol exposure on the levels of β-LH heteronuclear RNA (hnRNA). There was no significant difference detected in the level of β-LH hnRNA after ethanol exposure. Polysome distribution analysis was used to evaluate the association and disassociation of β-LH mRNA with polyribosomes since non-polyribosome associated mRNA may be more vulnerable to degradation by RNAases. The results indicated a decrease in the association of the β-LH mRNA with polysomes following acute ethanol exposure. This decrease in polyribosome association would increase the exposure of the β-LH transcript making it more susceptible to RNases. We conclude that the decrease in steady-state β-LH mRNA levels after ethanol exposure occurs because of increasing degradation of the transcript rendered vulnerable by displacement from polysomes and not through a decreased transcriptional rate.

Original languageEnglish (US)
Pages (from-to)469-473
Number of pages5
JournalEndocrine
Volume3
Issue number6
DOIs
StatePublished - Jun 1 1995

Keywords

  • ethanol
  • LH
  • mRNA
  • ribosomes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Further characterization of the impact of ethanol on βLH: alterations in polyribosome association of βLH mRNA'. Together they form a unique fingerprint.

  • Cite this