Further characterization of the murine collagenase (type IVB) gene promoter and analysis of mRNA expression in murine tissues

Julie Roach, Sun Jin Choi, Rebecca L. Schaub, Robin J. Leach, G. David Roodman, Sakamuri V. Reddy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The collagenase B type IV (Co14B) gene is highly expressed in the osteoclast, the primary bone-resorbing cell. However, factors that regulate expression of the Co14B gene are not well characterized. A murine P1 genomic clone containing a 94 kb sequence insert which contains the Co14B gene was isolated. A 4 kb EcoR1 DNA fragment containing the 5' flanking sequence of the gene was further subcloned and restriction mapped. Putative transcription factors such as SRY, Lyf-1, and GATA1 and 2, binding motifs were identified by sequence analysis in this promoter region. Enhancer and suppressor regions were mapped by transient expression of Co14B gene promoter deletion mutant-luciferase reporter gene constructs in HepG2 cells. Co14B mRNA expression in different murine tissues was analyzed by reverse transcription-polymerase chain reaction and demonstrated high levels of expression in bone, clavaria, spleen and thymus. This promoter provides a valuable tool for targeting gene expression to the osteoclast.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalGene
Volume208
Issue number2
DOIs
StatePublished - Feb 22 1998
Externally publishedYes

Fingerprint

Collagenases
Osteoclasts
Messenger RNA
Genes
Gene Expression
Bone and Bones
5' Flanking Region
Gene Deletion
Hep G2 Cells
Luciferases
Reporter Genes
Genetic Promoter Regions
Thymus Gland
Reverse Transcription
Sequence Analysis
Transcription Factors
Spleen
Clone Cells
Polymerase Chain Reaction
DNA

Keywords

  • Collagenase
  • Osteoclast
  • Promoter
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

Cite this

Further characterization of the murine collagenase (type IVB) gene promoter and analysis of mRNA expression in murine tissues. / Roach, Julie; Jin Choi, Sun; L. Schaub, Rebecca; Leach, Robin J.; Roodman, G. David; Reddy, Sakamuri V.

In: Gene, Vol. 208, No. 2, 22.02.1998, p. 117-122.

Research output: Contribution to journalArticle

Roach, Julie ; Jin Choi, Sun ; L. Schaub, Rebecca ; Leach, Robin J. ; Roodman, G. David ; Reddy, Sakamuri V. / Further characterization of the murine collagenase (type IVB) gene promoter and analysis of mRNA expression in murine tissues. In: Gene. 1998 ; Vol. 208, No. 2. pp. 117-122.
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