Further characterization of the murine collagenase (type IVB) gene promoter and analysis of mRNA expression in murine tissues

Julie Roach, Sun Jin Choi, Rebecca L. Schaub, Robin J. Leach, G. David Roodman, Sakamuri V. Reddy

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The collagenase B type IV (Co14B) gene is highly expressed in the osteoclast, the primary bone-resorbing cell. However, factors that regulate expression of the Co14B gene are not well characterized. A murine P1 genomic clone containing a 94 kb sequence insert which contains the Co14B gene was isolated. A 4 kb EcoR1 DNA fragment containing the 5' flanking sequence of the gene was further subcloned and restriction mapped. Putative transcription factors such as SRY, Lyf-1, and GATA1 and 2, binding motifs were identified by sequence analysis in this promoter region. Enhancer and suppressor regions were mapped by transient expression of Co14B gene promoter deletion mutant-luciferase reporter gene constructs in HepG2 cells. Co14B mRNA expression in different murine tissues was analyzed by reverse transcription-polymerase chain reaction and demonstrated high levels of expression in bone, clavaria, spleen and thymus. This promoter provides a valuable tool for targeting gene expression to the osteoclast.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalGene
Volume208
Issue number2
DOIs
StatePublished - Feb 22 1998

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Keywords

  • Collagenase
  • Osteoclast
  • Promoter
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

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