GABAergic and asymmetrical synapses on somata of GABAergic neurons in CA1 and CA3 regions of rat hippocampus: A quantitative electron microscopic analysis

Zhi B. Yao, Xiaoda Li, Zao C. Xu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and Purpose: CA1 pyramidal neurons in hippocampus die while CA3 neurons survive after transient ischemia. The imbalance of excitation and inhibition may contribute to this selective vulnerability. The purpose of this study was to examine the morphological basis of the above hypothesis. Methods: Male Wistar rats were perfused with 4% paraformaldehyde and 0.2% glutaraldehyde in 0.15 mol/L phosphate buffer. Coronal sections (50 μm) cut on a microtome were processed for γ-aminobutyric acid (GABA) immunocytochemistry. Sections for electron microscopy were postfixed in 0.5% osmium tetroxide and embedded in high-viscosity epoxy resin. Ultrathin sections were cut and observed with an electron microscope. Results: GABA- positive neurons in the stratum pyramidale received more GABAergic synapses than asymmetrical synapses. The percentage of somatic membrane of GABA positive neurons covered by asymmetrical synapses in the CA1 region (3.17±1.13%) was higher than that in the CA3 region (2.15±0.18%, P

Original languageEnglish (US)
Pages (from-to)1411-1416
Number of pages6
JournalStroke
Volume27
Issue number8
StatePublished - Aug 1996
Externally publishedYes

Fingerprint

GABAergic Neurons
Carisoprodol
Synapses
Hippocampus
Electrons
Osmium Tetroxide
Epoxy Resins
Aminobutyrates
Pyramidal Cells
Glutaral
Viscosity
gamma-Aminobutyric Acid
Wistar Rats
Electron Microscopy
Buffers
Ischemia
Immunohistochemistry
Phosphates
Neurons
Membranes

Keywords

  • cerebral ischemia
  • excitotoxicity
  • GABA
  • rats
  • selective vulnerability

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

GABAergic and asymmetrical synapses on somata of GABAergic neurons in CA1 and CA3 regions of rat hippocampus : A quantitative electron microscopic analysis. / Yao, Zhi B.; Li, Xiaoda; Xu, Zao C.

In: Stroke, Vol. 27, No. 8, 08.1996, p. 1411-1416.

Research output: Contribution to journalArticle

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AU - Li, Xiaoda

AU - Xu, Zao C.

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