Galectin-1 and galectin-3 in chronic pancreatitis

Li Wang, Helmut Friess, Zhaowen Zhu, Luciano Frigeri, Arthur Zimmermann, Murray Korc, Pascal O. Berberat, Markus W. Büchler

Research output: Contribution to journalArticle

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Abstract

Galectin-1 and galectin-3 have important functions in cell-cell interactions, cell adhesion to extracellular matrix, the organization of extracellular matrix, and tissue remodeling. To assess their potential role in chronic pancreatitis (CP), we examined their expression by Northern blot analysis, in situ hybridization, immunohistochemistry, and Western blot analysis in normal and CP pancreatic tissues. Northern blot analysis revealed a 4.5-fold increase of galectin-1 mRNA (p <0.01) and a 3.8-fold increase of galectin-3 mRNA (p <0.01) in CP samples compared with normal controls. In situ hybridization analysis of normal pancreas indicated low abundance of galectin-1 mRNA in fibroblasts, whereas galectin-3 mRNA was moderately present in ductal cells. CP samples exhibited moderate to intense galectin-1 mRNA signals in fibroblasts, whereas galectin-3 mRNA signals were intense in the ceils of ductular complexes and weak in the degenerating acinar cells, in addition, intense galectin-1 and galectin-3 mRNA signals were present in nerves of normal and CP samples. Immunohistochemistry showed a distribution pattern of galectin-1 and galectin-3 similar to that described for in situ hybridization. Relative quantification of galectin-1 and galectin-3 protein by immunoblotting revealed an increase of 3.2-fold and 3.0-fold, respectively, in CP compared with normal controls. There was a significant correlation between galectin-1 and fibrosis and between galectin-3 and fibrosis and the density of ductular complexes. Up-regulation of galectin-1 in fibroblasts and galectin-3 in ductular complexes suggests a role of these lectins in tissue remodeling in CP. Galectin-1 might participate in ECM Changes, whereas galectin-3 seems to be involved in both ECM changes and ductular complex formation.

Original languageEnglish (US)
Pages (from-to)1233-1241
Number of pages9
JournalLaboratory Investigation
Volume80
Issue number8
StatePublished - 2000
Externally publishedYes

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Galectin 1
Galectin 3
Chronic Pancreatitis
Messenger RNA
In Situ Hybridization
Fibroblasts
Northern Blotting
Extracellular Matrix
Fibrosis
Immunohistochemistry
Acinar Cells
Immunoblotting
Lectins
Cell Adhesion
Cell Communication
Pancreas
Up-Regulation
Western Blotting

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Wang, L., Friess, H., Zhu, Z., Frigeri, L., Zimmermann, A., Korc, M., ... Büchler, M. W. (2000). Galectin-1 and galectin-3 in chronic pancreatitis. Laboratory Investigation, 80(8), 1233-1241.

Galectin-1 and galectin-3 in chronic pancreatitis. / Wang, Li; Friess, Helmut; Zhu, Zhaowen; Frigeri, Luciano; Zimmermann, Arthur; Korc, Murray; Berberat, Pascal O.; Büchler, Markus W.

In: Laboratory Investigation, Vol. 80, No. 8, 2000, p. 1233-1241.

Research output: Contribution to journalArticle

Wang, L, Friess, H, Zhu, Z, Frigeri, L, Zimmermann, A, Korc, M, Berberat, PO & Büchler, MW 2000, 'Galectin-1 and galectin-3 in chronic pancreatitis', Laboratory Investigation, vol. 80, no. 8, pp. 1233-1241.
Wang L, Friess H, Zhu Z, Frigeri L, Zimmermann A, Korc M et al. Galectin-1 and galectin-3 in chronic pancreatitis. Laboratory Investigation. 2000;80(8):1233-1241.
Wang, Li ; Friess, Helmut ; Zhu, Zhaowen ; Frigeri, Luciano ; Zimmermann, Arthur ; Korc, Murray ; Berberat, Pascal O. ; Büchler, Markus W. / Galectin-1 and galectin-3 in chronic pancreatitis. In: Laboratory Investigation. 2000 ; Vol. 80, No. 8. pp. 1233-1241.
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