Garlic oil polysulfides: H2s-and o2-independent prooxidants in buffer and antioxidants in cells

Eric R. Deleon, Yan Gao, Evelyn Huang, Kenneth Olson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The health benefits of garlic and other organosulfur-containing foods are well recognized and have been attributed to both prooxidant and antioxidant activities. The effects of garlic are surprisingly similar to those of hydrogen sulfide (H2S), which is also known to be released from garlic under certain conditions. However, recent evidence suggests that polysulfides, not H2S, may be the actual mediator of physiological signaling. In this study, we monitored formation of H2S and polysulfides from garlic oil in buffer and in human embryonic kidney (HEK) 293 cells with fluorescent dyes, 7-azido-4-methylcoumarin and SSP4, respectively and redox activity with two redox indicators redox-sensitive green fluorescent protein (roGFP) and DCF. Our results show that H2S release from garlic oil in buffer requires other low-molecular-weight thiols, such as cysteine (Cys) or glutathione (GSH), whereas polysulfides are readily detected in garlic oil alone. Administration of garlic oil to cells rapidly increases intracellular polysulfide but has minimal effects on H2S unless Cys or GSH are also present in the extracellular medium. We also observed that garlic oil and diallyltrisulfide (DATS) potently oxidized roGFP in buffer but did not affect DCF. This appears to be a direct polysulfide-mediated oxidation that does not require a reactive oxygen species intermediate. Conversely, when applied to cells, garlic oil became a significant intracellular reductant independent of extracellular Cys or GSH. This suggests that intracellular metabolism and further processing of the sulfur moieties are necessary to confer antioxidant properties to garlic oil in vivo.

Original languageEnglish (US)
Pages (from-to)R1212-R1225
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume310
Issue number11
DOIs
StatePublished - Jun 1 2016

Fingerprint

Buffers
Antioxidants
Garlic
Oxidation-Reduction
Cysteine
Green Fluorescent Proteins
Hydrogen Sulfide
Reducing Agents
Insurance Benefits
polysulfide
allyl sulfide
Fluorescent Dyes
Sulfur
Sulfhydryl Compounds
Glutathione
Reactive Oxygen Species
Molecular Weight
Kidney
Food

Keywords

  • Allium
  • Dialyltrisulfide
  • Polysulfides

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)
  • Physiology (medical)

Cite this

Garlic oil polysulfides : H2s-and o2-independent prooxidants in buffer and antioxidants in cells. / Deleon, Eric R.; Gao, Yan; Huang, Evelyn; Olson, Kenneth.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 310, No. 11, 01.06.2016, p. R1212-R1225.

Research output: Contribution to journalArticle

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