GATA-1 deficiency rescues trabecular but not cortical bone in OPG deficient mice

Tomas E. Meijome, R. Adam Hooker, Ying Hua Cheng, Whitney Walker, Mark C. Horowitz, Robyn K. Fuchs, Melissa A. Kacena

Research output: Contribution to journalArticle

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Abstract

GATA-1<sup>low/low</sup> mice have an increase in megakaryocytes (MKs) and trabecular bone. The latter is thought to result from MKs directly stimulating osteoblastic bone formation while simultaneously inhibiting osteoclastogenesis. Osteoprotegerin (OPG) is known to inhibit osteoclastogenesis and OPG<sup>-/-</sup> mice have reduced trabecular and cortical bone due to increased osteoclastogenesis. Interestingly, GATA-1<sup>low/low</sup> mice have increased OPG levels. Here, we sought to determine whether GATA-1 knockdown in OPG<sup>-/-</sup> mice could rescue the observed osteoporotic bone phenotype. GATA-1<sup>low/low</sup> mice were bred with OPG<sup>-/-</sup> mice and bone phenotype assessed. GATA-1<sup>low/low</sup>×OPG<sup>-/-</sup> mice have increased cortical bone porosity, similar to OPG<sup>-/-</sup> mice. Both OPG<sup>-/-</sup> and GATA-1<sup>low/low</sup>×OPG<sup>-/-</sup> mice, were found to have increased osteoclasts localized to cortical bone, possibly producing the observed elevated porosity. Biomechanical assessment indicates that OPG<sup>-/-</sup> and GATA-1<sup>low/low</sup>×OPG<sup>-/-</sup> femurs are weaker and less stiff than C57BL/6 or GATA-1<sup>low/low</sup> femurs. Notably, GATA-1<sup>low/low</sup>×OPG<sup>-/-</sup> mice had trabecular bone parameters that were not different from C57BL/6 values, suggesting that GATA-1 deficiency can partially rescue the trabecular bone loss observed with OPG deficiency. The fact that GATA-1 deficiency appears to be able to partially rescue the trabecular, but not the cortical bone phenotype suggests that MKs can locally enhance trabecular bone volume, but that MK secreted factors cannot access cortical bone sufficiently to inhibit osteoclastogenesis or that OPG itself is required to inhibit osteoclastogenesis in cortical bone.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalJournal of cellular physiology
Volume230
Issue number4
DOIs
StatePublished - Apr 1 2015

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Osteoprotegerin
Bone
Osteogenesis
Megakaryocytes
Porosity
Phenotype
Femur
Cortical Bone
Bone and Bones
Osteoclasts
Cancellous Bone

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Meijome, T. E., Hooker, R. A., Cheng, Y. H., Walker, W., Horowitz, M. C., Fuchs, R. K., & Kacena, M. A. (2015). GATA-1 deficiency rescues trabecular but not cortical bone in OPG deficient mice. Journal of cellular physiology, 230(4), 783-790. https://doi.org/10.1002/jcp.24803

GATA-1 deficiency rescues trabecular but not cortical bone in OPG deficient mice. / Meijome, Tomas E.; Hooker, R. Adam; Cheng, Ying Hua; Walker, Whitney; Horowitz, Mark C.; Fuchs, Robyn K.; Kacena, Melissa A.

In: Journal of cellular physiology, Vol. 230, No. 4, 01.04.2015, p. 783-790.

Research output: Contribution to journalArticle

Meijome, TE, Hooker, RA, Cheng, YH, Walker, W, Horowitz, MC, Fuchs, RK & Kacena, MA 2015, 'GATA-1 deficiency rescues trabecular but not cortical bone in OPG deficient mice', Journal of cellular physiology, vol. 230, no. 4, pp. 783-790. https://doi.org/10.1002/jcp.24803
Meijome TE, Hooker RA, Cheng YH, Walker W, Horowitz MC, Fuchs RK et al. GATA-1 deficiency rescues trabecular but not cortical bone in OPG deficient mice. Journal of cellular physiology. 2015 Apr 1;230(4):783-790. https://doi.org/10.1002/jcp.24803
Meijome, Tomas E. ; Hooker, R. Adam ; Cheng, Ying Hua ; Walker, Whitney ; Horowitz, Mark C. ; Fuchs, Robyn K. ; Kacena, Melissa A. / GATA-1 deficiency rescues trabecular but not cortical bone in OPG deficient mice. In: Journal of cellular physiology. 2015 ; Vol. 230, No. 4. pp. 783-790.
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