Gender- and age-dependent changes in kidney androgen protein mRNA expression in a knockout mouse model for nephrolithiasis

Eleni G. Tzortzaki, Dayna Glass, Min Yang, Andrew Evan, Sharon B. Bledsoe, Peter J. Stambrook, Amrik Sahota, Jay A. Tischfield

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Kidney androgen-regulated protein (Kap) is the most abundant protein in the mouse kidney, but its function is unknown. We previously observed a significant decrease in Kap mRNA expression in whole kidney tissue from male mice with adenine phosphoribosyltransferase (APRT) deficiency and 2,8-dihydroxyadenine (DHA) nephrolithiasis. The disease phenotype is more severe in male mice and is age-dependent. To identify the cellular basis for differential Kap expression, we used in situ hybridization (ISH) and reverse transcription-polymerase chain reaction ISH (RT-PCR ISH) to identify the cell types expressing this mRNA in paraffin-embedded kidney sections. In 1-month-old wild-type male mice, Kap was detected primarily in S3 proximal tubule segments, but expression was very low in female mice. In 1-month-old APRT-deficient male mice, Kap expression was decreased significantly and was undetectable in female mice. Kap mRNA was not detected in 3- or 6-month-old mice using our standard ISH protocol, but we observed intense cytoplasmic staining in S3 proximal tubules in wild-type male mice of these age groups using an improved RT-PCR ISH procedure. Our studies demonstrate age-, gender-, and APRT genotype-dependent changes in Kap mRNA expression in mouse kidney. Kap expression is under multihormonal control, and hormonal changes in DHA-induced nephrolithiasis may account for the decreased Kap expression in APRT-deficient mice.

Original languageEnglish
Pages (from-to)1663-1669
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume50
Issue number12
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Nephrolithiasis
Knockout Mice
Androgens
Kidney
Messenger RNA
Adenine Phosphoribosyltransferase
Proteins
In Situ Hybridization
Reverse Transcription
Polymerase Chain Reaction
Paraffin
Age Groups
Genotype

Keywords

  • 2,8-dihydroxadenine nephrolithiasis
  • Adenine phosphoribosyltransferase deficiency
  • In situ hybridization
  • Kidney androgen-regulated protein
  • Reverse transcription-polymerase chain reaction in situ hybridization

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Tzortzaki, E. G., Glass, D., Yang, M., Evan, A., Bledsoe, S. B., Stambrook, P. J., ... Tischfield, J. A. (2002). Gender- and age-dependent changes in kidney androgen protein mRNA expression in a knockout mouse model for nephrolithiasis. Journal of Histochemistry and Cytochemistry, 50(12), 1663-1669.

Gender- and age-dependent changes in kidney androgen protein mRNA expression in a knockout mouse model for nephrolithiasis. / Tzortzaki, Eleni G.; Glass, Dayna; Yang, Min; Evan, Andrew; Bledsoe, Sharon B.; Stambrook, Peter J.; Sahota, Amrik; Tischfield, Jay A.

In: Journal of Histochemistry and Cytochemistry, Vol. 50, No. 12, 01.12.2002, p. 1663-1669.

Research output: Contribution to journalArticle

Tzortzaki, EG, Glass, D, Yang, M, Evan, A, Bledsoe, SB, Stambrook, PJ, Sahota, A & Tischfield, JA 2002, 'Gender- and age-dependent changes in kidney androgen protein mRNA expression in a knockout mouse model for nephrolithiasis', Journal of Histochemistry and Cytochemistry, vol. 50, no. 12, pp. 1663-1669.
Tzortzaki, Eleni G. ; Glass, Dayna ; Yang, Min ; Evan, Andrew ; Bledsoe, Sharon B. ; Stambrook, Peter J. ; Sahota, Amrik ; Tischfield, Jay A. / Gender- and age-dependent changes in kidney androgen protein mRNA expression in a knockout mouse model for nephrolithiasis. In: Journal of Histochemistry and Cytochemistry. 2002 ; Vol. 50, No. 12. pp. 1663-1669.
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