Gender difference in regulation of the branched-chain a-keto acid dehydrogenase complex (bckdc) and its kinase

R. Kobayashi, Y. Shimomura, T. Murakami, N. Nakai, M. Otsuka, X. Arakawa, K. M. Popov, Robert Harris

Research output: Contribution to journalArticle

Abstract

Regulation of the activity state of the hepatic BCKDC during the light-dark cycle differs markedly in male and female rats. Male rats show little evidence of a diurnal rhythm in the regulation of the complex. However, female rats exhibit a profound diurnal rhythm in the activity state. Regardless of gender most of the complex was dephosphorylated and active in the middle of the dark period and early in the light period, and this form of the complex predominated in male rats al the end of the light period. In contrast, most of the complex in female rats became phosphorylated and inactive by the end of the light period. Changes in blood levels of branched-chain o-keto acids, only known regulators of BCKDH kinase, did not correlate with changes in BCKDC activity state in female rats. Rather it appears that the greater BCKDC phosphorylation is due to a stable increase in BCKDH kinase activity. Western blot analysis indicates that the increased expression of BCKDH kinase protein is responsible for the increase in BCKDH kinase activity. Thus, this study provides evidence for a gender-specific difference in the regulation of branched chain amino acid <atul>olisni. A diurnal variation in expression of BCKDH kinase appears to be ;in important factor in the difference in the regulation of BCKDC1 activity between male and female rats.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997
Externally publishedYes

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3-methyl-2-oxobutanoate dehydrogenase (lipoamide)
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Keto Acids
gender differences
Rats
Oxidoreductases
phosphotransferases (kinases)
Phosphotransferases
rats
photophase
Circadian Rhythm
Light
circadian rhythm
keto acids
Branched Chain Amino Acids
Phosphorylation
branched chain amino acids
gender
Photoperiod
scotophase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kobayashi, R., Shimomura, Y., Murakami, T., Nakai, N., Otsuka, M., Arakawa, X., ... Harris, R. (1997). Gender difference in regulation of the branched-chain a-keto acid dehydrogenase complex (bckdc) and its kinase. FASEB Journal, 11(9).

Gender difference in regulation of the branched-chain a-keto acid dehydrogenase complex (bckdc) and its kinase. / Kobayashi, R.; Shimomura, Y.; Murakami, T.; Nakai, N.; Otsuka, M.; Arakawa, X.; Popov, K. M.; Harris, Robert.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Kobayashi, R, Shimomura, Y, Murakami, T, Nakai, N, Otsuka, M, Arakawa, X, Popov, KM & Harris, R 1997, 'Gender difference in regulation of the branched-chain a-keto acid dehydrogenase complex (bckdc) and its kinase', FASEB Journal, vol. 11, no. 9.
Kobayashi R, Shimomura Y, Murakami T, Nakai N, Otsuka M, Arakawa X et al. Gender difference in regulation of the branched-chain a-keto acid dehydrogenase complex (bckdc) and its kinase. FASEB Journal. 1997;11(9).
Kobayashi, R. ; Shimomura, Y. ; Murakami, T. ; Nakai, N. ; Otsuka, M. ; Arakawa, X. ; Popov, K. M. ; Harris, Robert. / Gender difference in regulation of the branched-chain a-keto acid dehydrogenase complex (bckdc) and its kinase. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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