Gene and metabolite time-course response to cigarette smoking in mouse lung and plasma

Mikaela A. Miller, Thomas Danhorn, Charmion I. Cruickshank-Quinn, Sonia M. Leach, Sean Jacobson, Matthew J. Strand, Nichole A. Reisdorph, Russell P. Bowler, Irina Petrache, Katerina Kechris

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Prolonged cigarette smoking (CS) causes chronic obstructive pulmonary disease (COPD), a prevalent serious condition that may persist or progress after smoking cessation. To provide insight into how CS triggers COPD, we investigated temporal patterns of lung transcriptome expression and systemic metabolome changes induced by chronic CS exposure and smoking cessation. Whole lung RNA-seq data was analyzed at transcript and exon levels from C57Bl/6 mice exposed to CS for 1- or 7 days, for 3-, 6-, or 9 months, or for 6 months followed by 3 months of cessation using age-matched littermate controls. We identified previously unreported dysregulation of pyrimidine metabolism and phosphatidylinositol signaling pathways and confirmed alterations in glutathione metabolism and circadian gene pathways. Almost all dysregulated pathways demonstrated reversibility upon smoking cessation, except the lysosome pathway. Chronic CS exposure was significantly linked with alterations in pathways encoding for energy, phagocytosis, and DNA repair and triggered differential expression of genes or exons previously unreported to associate with CS or COPD, including Lox, involved in matrix remodeling, Gp2, linked to goblet cells, and Slc22a12 and Agpat3, involved in purine and glycerolipid metabolism, respectively. CSinduced lung metabolic pathways changes were validated using metabolomic profiles of matched plasma samples, indicating that dynamic metabolic gene regulation caused by CS is reflected in the plasma metabolome. Using advanced technologies, our study uncovered novel pathways and genes altered by chronic CS exposure, including those involved in pyrimidine metabolism, phosphatidylinositol signaling and lysosome function, highlighting their potential importance in the pathogenesis or diagnosis of CS-associated conditions.

Original languageEnglish (US)
Article numbere0178281
JournalPLoS One
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2017

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smoking (habit)
Metabolites
Tobacco Products
Genes
Smoking
lungs
metabolites
Plasmas
Lung
mice
Metabolism
Pulmonary diseases
genes
smoking (food products)
Smoking Cessation
respiratory tract diseases
Chronic Obstructive Pulmonary Disease
metabolome
metabolism
Metabolome

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Miller, M. A., Danhorn, T., Cruickshank-Quinn, C. I., Leach, S. M., Jacobson, S., Strand, M. J., ... Kechris, K. (2017). Gene and metabolite time-course response to cigarette smoking in mouse lung and plasma. PLoS One, 12(6), [e0178281]. https://doi.org/10.1371/journal.pone.0178281

Gene and metabolite time-course response to cigarette smoking in mouse lung and plasma. / Miller, Mikaela A.; Danhorn, Thomas; Cruickshank-Quinn, Charmion I.; Leach, Sonia M.; Jacobson, Sean; Strand, Matthew J.; Reisdorph, Nichole A.; Bowler, Russell P.; Petrache, Irina; Kechris, Katerina.

In: PLoS One, Vol. 12, No. 6, e0178281, 01.06.2017.

Research output: Contribution to journalArticle

Miller, MA, Danhorn, T, Cruickshank-Quinn, CI, Leach, SM, Jacobson, S, Strand, MJ, Reisdorph, NA, Bowler, RP, Petrache, I & Kechris, K 2017, 'Gene and metabolite time-course response to cigarette smoking in mouse lung and plasma', PLoS One, vol. 12, no. 6, e0178281. https://doi.org/10.1371/journal.pone.0178281
Miller MA, Danhorn T, Cruickshank-Quinn CI, Leach SM, Jacobson S, Strand MJ et al. Gene and metabolite time-course response to cigarette smoking in mouse lung and plasma. PLoS One. 2017 Jun 1;12(6). e0178281. https://doi.org/10.1371/journal.pone.0178281
Miller, Mikaela A. ; Danhorn, Thomas ; Cruickshank-Quinn, Charmion I. ; Leach, Sonia M. ; Jacobson, Sean ; Strand, Matthew J. ; Reisdorph, Nichole A. ; Bowler, Russell P. ; Petrache, Irina ; Kechris, Katerina. / Gene and metabolite time-course response to cigarette smoking in mouse lung and plasma. In: PLoS One. 2017 ; Vol. 12, No. 6.
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