Gene-based approaches for the repair of articular cartilage

Stephen Trippel, S. C. Ghivizzani, A. J. Nixon

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Gene transfer technology has opened novel treatment avenues toward the treatment of damaged musculoskeletal tissues, and may be particularly beneficial to articular cartilage. There is no natural repair mechanism to heal damaged or diseased cartilage. Existing pharmacologic, surgical and cell based treatments may offer temporary relief but are incapable of restoring damaged cartilage to its normal phenotype. Gene transfer provides the capability to achieve sustained, localized presentation of bioactive proteins or gene products to sites of tissue damage. A variety of cDNAs have been cloned which may be used to stimulate biological processes that could improve cartilage healing by (1) inducing mitosis and the synthesis and deposition of cartilage extracellular matrix components by chondrocytes, (2) induction of chondrogenesis by mesenchymal progenitor cells, or (3) inhibiting cellular responses to inflammatory stimuli. The challenge is to adapt this technology into a useful clinical treatment modality. Using different marker genes, the principle of gene delivery to synovium, chondrocytes and mesenchymal progenitor cells has been convincingly demonstrated. Following this, research efforts have begun to move to functional studies. This involves the identification of appropriate gene or gene combinations, incorporation of these cDNAs into appropriate vectors and delivery to specific target cells within the proper biological context to achieve a meaningful therapeutic response. Methods currently being explored range from those as simple as direct delivery of a vector to a cartilage defect, to synthesis of cartilaginous implants through gene-enhanced tissue engineering. Data from recent efficacy studies provide optimism that gene delivery can be harnessed to guide biological processes toward both accelerated and improved articular cartilage repair.

Original languageEnglish
Pages (from-to)351-359
Number of pages9
JournalGene Therapy
Volume11
Issue number4
DOIs
StatePublished - Feb 2004

Fingerprint

Articular Cartilage
Genes
Cartilage
Biological Phenomena
Chondrocytes
Mesenchymal Stromal Cells
Complementary DNA
Cartilage Diseases
Technology Transfer
Chondrogenesis
Synovial Membrane
Tissue Engineering
Mitosis
Extracellular Matrix
Technology
Phenotype
Research

Keywords

  • Articular cartilage
  • Chondrocyte
  • Gene delivery
  • Growth factor
  • Mesenchymal stem cell

ASJC Scopus subject areas

  • Genetics

Cite this

Trippel, S., Ghivizzani, S. C., & Nixon, A. J. (2004). Gene-based approaches for the repair of articular cartilage. Gene Therapy, 11(4), 351-359. https://doi.org/10.1038/sj.gt.3302201

Gene-based approaches for the repair of articular cartilage. / Trippel, Stephen; Ghivizzani, S. C.; Nixon, A. J.

In: Gene Therapy, Vol. 11, No. 4, 02.2004, p. 351-359.

Research output: Contribution to journalArticle

Trippel, S, Ghivizzani, SC & Nixon, AJ 2004, 'Gene-based approaches for the repair of articular cartilage', Gene Therapy, vol. 11, no. 4, pp. 351-359. https://doi.org/10.1038/sj.gt.3302201
Trippel, Stephen ; Ghivizzani, S. C. ; Nixon, A. J. / Gene-based approaches for the repair of articular cartilage. In: Gene Therapy. 2004 ; Vol. 11, No. 4. pp. 351-359.
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