Gene expression and atrial natriuretic factor processing and secretion in cultured at-1 cardiac myocytes

Nicholas A. Lanson, Christopher C. Glembotski, Mark E. Steinhelper, Loren Field, William C. Claycomb

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background. Studies were carried out to characterize several biochemical features of cultured AT-1 cells. Methods and Results. These cells were obtained from a transplantable atrial cardiomyocyte tumor lineage. Reverse transcriptase-polymerase chain reaction-based analyses demonstrated that the pattern of gene expression of cultured AT-1 cells was similar to that of adult atrial myocytes. AT-1 cells expressed atrial natriuretic factor (ANF), α-cardiac myosin heavy chain, α-cardiac actin, and connexin43. Radioimmunoassays verified that the cells synthesized, stored, and secreted ANF. Through size-exclusion, reversed-phase, and carboxymethyl-ion-exchange high-performance liquid chromatography, it was shown that cultured AT-1 cells stored ANF as pro-ANF (ANF-[1-126]), which was cosecretionally processed quantitatively to ANF-(1-98) and the bioactive 28-amino-acid ANF-(99-126). In addition, cultured AT-1 cells secreted ANF at almost a sixfold greater rate in response to endothelin-1, a potent secretagogue of ANF. KCl, metenkephalinamide, isoproterenol, phenylephrine, and 12-O-tetradecanoyl-phorbol-13-acetate also stimulated ANF release. Conclusions. These studies, in combination with previous findings, demonstrated that cultured AT-1 cells, while maintaining the ability to proliferate, have retained functional, biochemical, and ultrastructural features that are characteristic of adult atrial myocytes.

Original languageEnglish
Pages (from-to)1835-1841
Number of pages7
JournalCirculation
Volume85
Issue number5
StatePublished - May 1992

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Atrial Natriuretic Factor
Cardiac Myocytes
Gene Expression
Muscle Cells
Cardiac Myosins
Connexin 43
Myosin Heavy Chains
Ion Exchange
Gene Expression Profiling
Phenylephrine
Endothelin-1
Tetradecanoylphorbol Acetate
Reverse Transcriptase Polymerase Chain Reaction
Isoproterenol
Radioimmunoassay
Actins
High Pressure Liquid Chromatography
Amino Acids

Keywords

  • Cardiac myocyte hyperplasia
  • Cell culture
  • SV40 T antigen
  • Transgenic mice

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Lanson, N. A., Glembotski, C. C., Steinhelper, M. E., Field, L., & Claycomb, W. C. (1992). Gene expression and atrial natriuretic factor processing and secretion in cultured at-1 cardiac myocytes. Circulation, 85(5), 1835-1841.

Gene expression and atrial natriuretic factor processing and secretion in cultured at-1 cardiac myocytes. / Lanson, Nicholas A.; Glembotski, Christopher C.; Steinhelper, Mark E.; Field, Loren; Claycomb, William C.

In: Circulation, Vol. 85, No. 5, 05.1992, p. 1835-1841.

Research output: Contribution to journalArticle

Lanson, NA, Glembotski, CC, Steinhelper, ME, Field, L & Claycomb, WC 1992, 'Gene expression and atrial natriuretic factor processing and secretion in cultured at-1 cardiac myocytes', Circulation, vol. 85, no. 5, pp. 1835-1841.
Lanson NA, Glembotski CC, Steinhelper ME, Field L, Claycomb WC. Gene expression and atrial natriuretic factor processing and secretion in cultured at-1 cardiac myocytes. Circulation. 1992 May;85(5):1835-1841.
Lanson, Nicholas A. ; Glembotski, Christopher C. ; Steinhelper, Mark E. ; Field, Loren ; Claycomb, William C. / Gene expression and atrial natriuretic factor processing and secretion in cultured at-1 cardiac myocytes. In: Circulation. 1992 ; Vol. 85, No. 5. pp. 1835-1841.
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