Gene expression profiles of lens regeneration and development in Xenopus laevis

Erica L. Malloch, Kimberly J. Perry, Lisa Fukui, Verity R. Johnson, Jason Wever, Caroline W. Beck, Michael W. King, Jonathan J. Henry

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Seven hundred and thirty-four unique genes were recovered from a cDNA library enriched for genes up-regulated during the process of lens regeneration in the frog Xenopus laevis. The sequences represent transcription factors, proteins involved in RNA synthesis/processing, components of prominent cell signaling pathways, genes involved in protein processing, transport, and degradation (e.g., the ubiquitin/proteasome pathway), matrix metalloproteases (MMPs), as well as many other proteins. The findings implicate specific signal transduction pathways in the process of lens regeneration, including the FGF, TGF-beta, MAPK, Retinoic acid, Wnt, and hedgehog signaling pathways, which are known to play important roles in eye/lens development and regeneration in various systems. In situ hybridization revealed that the majority of genes recovered are expressed during embryogenesis, including in eye tissues. Several novel genes specifically expressed in lenses were identified. The suite of genes was compared to those up-regulated in other regenerating tissues/organisms, and a small degree of overlap was detected.

Original languageEnglish (US)
Pages (from-to)2340-2356
Number of pages17
JournalDevelopmental Dynamics
Volume238
Issue number9
DOIs
StatePublished - Sep 21 2009

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Keywords

  • Development
  • Eye
  • Lens
  • Regeneration
  • Transdifferentiation
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Malloch, E. L., Perry, K. J., Fukui, L., Johnson, V. R., Wever, J., Beck, C. W., King, M. W., & Henry, J. J. (2009). Gene expression profiles of lens regeneration and development in Xenopus laevis. Developmental Dynamics, 238(9), 2340-2356. https://doi.org/10.1002/dvdy.21998