Gene expression profiling of mice with genetically modified muscle glycogen content

Gretchen E. Parker, Bartholomew A. Pederson, Mariko Obayashi, Jill M. Schroeder, Robert Harris, Peter Roach

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glycogen, a branched polymer of glucose, forms an energy reserve in numerous organisms. In mammals, the two largest glycogen stores are in skeletal muscle and liver, which express tissue-specific glycogen synthase isoforms. MGSKO mice, in which mGys1 (mouse glycogen synthase) is disrupted, are devoid of muscle glycogen [Pederson, Chen, Schroeder, Shou, DePaoli-Roach and Roach (2004) Mol. Cell. Biol. 24, 7179-7187]. The GSL30 mouse line hyper-accumulates glycogen in muscle [Manchester, Skurat, Roach, Hauschka and Lawrence (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 10707-10711]. We performed a microarray analysis of mRNA from the anterior tibialis, medial gastrocnemius and liver of MGSKO mice, and from the gastrocnemius of GSL30 mice. In MGSKO mice, transcripts of 79 genes varied in their expression in the same direction in both the anterior tibialis and gastrocnemius. These included several genes encoding proteins proximally involved in glycogen metabolism. The Ppp1r1a [protein phosphatase 1 regulatory (inhibitor) subunit 1A] gene underwent the greatest amount of downregulation. In muscle, the downregulation of Pfkfb1 and Pfkfb3, encoding isoforms of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, is consistent with decreased glycolysis. Pathways for branched-chain amino acid, and ketone body utilization appear to be downregulated, as is the capacity to form the gluconeogenic precursors alanine, lactate and glutamine. Expression changes among several members of the Wnt signalling pathway were identified, suggesting an as yet unexplained role in glycogen metabolism. In liver, the upregulation of Pfkfb1 and Pfkfb3 expression is consistent with increased glycolysis, perhaps as an adaptation to altered muscle metabolism. By comparing changes in muscle expression between MGSKO and GSL30 mice, we found a subset of 44 genes, the expression of which varied as a function of muscle glycogen content. These genes are candidates for regulation by glycogen levels. Particularly interesting is the observation that 11 of these genes encode cardiac or slow-twitch isoforms of muscle contractile proteins, and are upregulated in muscle that has a greater oxidative capacity in MGSKO mice.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalBiochemical Journal
Volume395
Issue number1
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

Fingerprint

Gene Expression Profiling
Glycogen
Gene expression
Muscle
Muscles
Genes
Metabolism
Liver
Glycogen Synthase
Protein Isoforms
Down-Regulation
Glycolysis
Phosphofructokinase-2
Contractile Proteins
Ketone Bodies
Branched Chain Amino Acids
Wnt Signaling Pathway
Glucans
Protein Phosphatase 1
Muscle Proteins

Keywords

  • Branched-chain amino acid oxidation
  • cAMP
  • Glycogen
  • Glycogen synthase
  • Phosphatase inhibitor 1 (I-1)
  • Wnt

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gene expression profiling of mice with genetically modified muscle glycogen content. / Parker, Gretchen E.; Pederson, Bartholomew A.; Obayashi, Mariko; Schroeder, Jill M.; Harris, Robert; Roach, Peter.

In: Biochemical Journal, Vol. 395, No. 1, 01.04.2006, p. 137-145.

Research output: Contribution to journalArticle

Parker, Gretchen E. ; Pederson, Bartholomew A. ; Obayashi, Mariko ; Schroeder, Jill M. ; Harris, Robert ; Roach, Peter. / Gene expression profiling of mice with genetically modified muscle glycogen content. In: Biochemical Journal. 2006 ; Vol. 395, No. 1. pp. 137-145.
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