Gene expression within the extended amygdala of 5 pairs of rat lines selectively bred for high or low ethanol consumption

William J. McBride, Mark W. Kimpel, Jeanette McClintick, Zheng Ming Ding, Petri Hyytia, Giancarlo Colombo, Tiebing Liang, Howard Edenberg, Lawrence Lumeng, Richard Bell

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The objectives of this study were to determine innate differences in gene expression in 2 regions of the extended amygdala between 5 different pairs of lines of male rats selectively bred for high or low ethanol consumption: a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats, b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line-pairs 1 and 2), c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats, and d) Sardinian alcohol-preferring (sP) vs. Sardinian alcohol-nonpreferring (sNP) rats, and then to determine if these differences are common across the line-pairs. Microarray analysis revealed up to 1772 unique named genes in the nucleus accumbens shell (AcbSh) and 494 unique named genes in the central nucleus of the amygdala (CeA) that significantly differed [False Discovery Rate (FDR)=0.10; fold-change at least 1.2] in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 or 4 line-pairs, but not for all 5 line-pairs. However, there were almost no individual genes in common within these categories and networks. ANOVAs of the combined data for the 5 line-pairs indicated 1014 and 731 significant (p<0.01) differences in expression of named genes in the AcbSh and CeA, respectively. There were 4-6 individual named genes that significantly differed across up to 3 line-pairs in both regions; only 1 gene (Gsta4 in the CeA) differed in as many as 4 line-pairs. Overall, the findings suggest that a) some biological categories or networks (e.g., cell-to-cell signaling, cellular stress response, cellular organization, etc.) may be in common for subsets of line-pairs within either the AcbSh or CeA, and b) regulation of different genes and/or combinations of multiple biological systems may be contributing to the disparate alcohol drinking behaviors of these line-pairs.

Original languageEnglish
Pages (from-to)517-529
Number of pages13
JournalAlcohol
Volume47
Issue number7
DOIs
StatePublished - Nov 2013

Fingerprint

Amygdala
Gene expression
Rats
Ethanol
Genes
alcohol
Alcohols
Gene Expression
Alcohol Drinking
Drinking Behavior
Gene Ontology
Nucleus Accumbens
Microarray Analysis
Cell signaling
Analysis of Variance
Biological systems
Microarrays
Analysis of variance (ANOVA)
ontology
Ontology

Keywords

  • Alcohol-preferring
  • Alko alcohol
  • Central nucleus of the amygdala
  • Gene expression
  • High-alcohol-drinking
  • Nucleus accumbens
  • Sardinian alcohol-preferring
  • Selectively bred rat lines

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Behavioral Neuroscience
  • Neurology
  • Toxicology
  • Health(social science)

Cite this

Gene expression within the extended amygdala of 5 pairs of rat lines selectively bred for high or low ethanol consumption. / McBride, William J.; Kimpel, Mark W.; McClintick, Jeanette; Ding, Zheng Ming; Hyytia, Petri; Colombo, Giancarlo; Liang, Tiebing; Edenberg, Howard; Lumeng, Lawrence; Bell, Richard.

In: Alcohol, Vol. 47, No. 7, 11.2013, p. 517-529.

Research output: Contribution to journalArticle

McBride, William J. ; Kimpel, Mark W. ; McClintick, Jeanette ; Ding, Zheng Ming ; Hyytia, Petri ; Colombo, Giancarlo ; Liang, Tiebing ; Edenberg, Howard ; Lumeng, Lawrence ; Bell, Richard. / Gene expression within the extended amygdala of 5 pairs of rat lines selectively bred for high or low ethanol consumption. In: Alcohol. 2013 ; Vol. 47, No. 7. pp. 517-529.
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