Gene structure and expression of phospholemman in mouse

Roberta C. Bogaev, Li Guo Jia, Yvonne M. Kobayashi, Cathy J. Palmer, J. Paul Mounsey, J. Randall Moorman, Larry R. Jones, Amy L. Tucker

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Phospholemman (PLM) is a small transmembrane cardiac protein that is the major sarcolemmal substrate for phosphorylation in response to adrenergic stimulation. PLM likely plays a role in muscle contractility and cell volume regulation through its function as a channel or a channel regulator. We are the first to describe the structure of the PLM gene and to demonstrate PLM cDNA splice variants. We cloned the murine PLM cDNA and used it as a probe to isolate the gene from a 129/SvJ genomic library. The gene contains seven introns and eight exons. The coding sequence is interrupted by five introns; the 5′ untranslated region by two. Using rapid amplification of 5′ cDNA ends we identified transcription start sites and four splice variants of the 5′ untranslated domain. There was no TATA box or CAAT box in the putative promoter regions. The gene has several stretches of dinucleotide repeats. The 3′ untranslated domains of mouse PLM cDNA clones show sequence differences not accounted for by alternative splicing. Mouse PLM shares 93, 83 and 80% amino acid identity with rat, dog, and human PLMs, respectively. Tissue expression of murine PLM parallels that in other species, being highest in heart, skeletal muscle, and liver.

Original languageEnglish (US)
Pages (from-to)69-79
Number of pages11
JournalGene
Volume271
Issue number1
DOIs
StatePublished - Jun 13 2001

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Keywords

  • Alternative splicing
  • Channel regulator
  • Chloride channel
  • Library screening
  • Northern blotting
  • Taurine

ASJC Scopus subject areas

  • Genetics

Cite this

Bogaev, R. C., Jia, L. G., Kobayashi, Y. M., Palmer, C. J., Mounsey, J. P., Moorman, J. R., Jones, L. R., & Tucker, A. L. (2001). Gene structure and expression of phospholemman in mouse. Gene, 271(1), 69-79. https://doi.org/10.1016/S0378-1119(01)00497-8