Gene structure and expression of phospholemman in mouse

Roberta C. Bogaev, LiGuo Jia, Yvonne M. Kobayashi, Cathy J. Palmer, J. Paul Mounsey, J. Randall Moorman, Larry Jones, Amy L. Tucker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Phospholemman (PLM) is a small transmembrane cardiac protein that is the major sarcolemmal substrate for phosphorylation in response to adrenergic stimulation. PLM likely plays a role in muscle contractility and cell volume regulation through its function as a channel or a channel regulator. We are the first to describe the structure of the PLM gene and to demonstrate PLM cDNA splice variants. We cloned the murine PLM cDNA and used it as a probe to isolate the gene from a 129/SvJ genomic library. The gene contains seven introns and eight exons. The coding sequence is interrupted by five introns; the 5′ untranslated region by two. Using rapid amplification of 5′ cDNA ends we identified transcription start sites and four splice variants of the 5′ untranslated domain. There was no TATA box or CAAT box in the putative promoter regions. The gene has several stretches of dinucleotide repeats. The 3′ untranslated domains of mouse PLM cDNA clones show sequence differences not accounted for by alternative splicing. Mouse PLM shares 93, 83 and 80% amino acid identity with rat, dog, and human PLMs, respectively. Tissue expression of murine PLM parallels that in other species, being highest in heart, skeletal muscle, and liver.

Original languageEnglish
Pages (from-to)69-79
Number of pages11
JournalGene
Volume271
Issue number1
DOIs
StatePublished - Jun 13 2001

Fingerprint

Gene Expression
Complementary DNA
Introns
Genes
Dinucleotide Repeats
TATA Box
Genomic Library
phospholemman
5' Untranslated Regions
Transcription Initiation Site
Alternative Splicing
Cell Size
Genetic Promoter Regions
Adrenergic Agents
Muscle Cells
Exons
Myocardium
Skeletal Muscle
Clone Cells
Phosphorylation

Keywords

  • Alternative splicing
  • Channel regulator
  • Chloride channel
  • Library screening
  • Northern blotting
  • Taurine

ASJC Scopus subject areas

  • Genetics

Cite this

Bogaev, R. C., Jia, L., Kobayashi, Y. M., Palmer, C. J., Mounsey, J. P., Moorman, J. R., ... Tucker, A. L. (2001). Gene structure and expression of phospholemman in mouse. Gene, 271(1), 69-79. https://doi.org/10.1016/S0378-1119(01)00497-8

Gene structure and expression of phospholemman in mouse. / Bogaev, Roberta C.; Jia, LiGuo; Kobayashi, Yvonne M.; Palmer, Cathy J.; Mounsey, J. Paul; Moorman, J. Randall; Jones, Larry; Tucker, Amy L.

In: Gene, Vol. 271, No. 1, 13.06.2001, p. 69-79.

Research output: Contribution to journalArticle

Bogaev, RC, Jia, L, Kobayashi, YM, Palmer, CJ, Mounsey, JP, Moorman, JR, Jones, L & Tucker, AL 2001, 'Gene structure and expression of phospholemman in mouse', Gene, vol. 271, no. 1, pp. 69-79. https://doi.org/10.1016/S0378-1119(01)00497-8
Bogaev RC, Jia L, Kobayashi YM, Palmer CJ, Mounsey JP, Moorman JR et al. Gene structure and expression of phospholemman in mouse. Gene. 2001 Jun 13;271(1):69-79. https://doi.org/10.1016/S0378-1119(01)00497-8
Bogaev, Roberta C. ; Jia, LiGuo ; Kobayashi, Yvonne M. ; Palmer, Cathy J. ; Mounsey, J. Paul ; Moorman, J. Randall ; Jones, Larry ; Tucker, Amy L. / Gene structure and expression of phospholemman in mouse. In: Gene. 2001 ; Vol. 271, No. 1. pp. 69-79.
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