Gene targets of mouse miR-709: Regulation of distinct pools

Sneha Surendran, Victoria N. Jideonwo, Chris Merchun, Miwon Ahn, John Murray, Jennifer Ryan, Kenneth Dunn, Janaiah Kota, Nuria Morral

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

MicroRNA (miRNA) are short non-coding RNA molecules that regulate multiple cellular processes, including development, cell differentiation, proliferation and death. Nevertheless, little is known on whether miRNA control the same gene networks in different tissues. miR-709 is an abundant miRNA expressed ubiquitously. Through transcriptome analysis, we have identified targets of miR-709 in hepatocytes. miR-709 represses genes implicated in cytoskeleton organization, extracellular matrix attachment, and fatty acid metabolism. Remarkably, none of the previously identified targets in non-hepatic tissues are silenced by miR-709 in hepatocytes, even though several of these genes are abundantly expressed in liver. In addition, miR-709 is upregulated in hepatocellular carcinoma, suggesting it participates in the genetic reprogramming that takes place during cell division, when cytoskeleton remodeling requires substantial changes in gene expression. In summary, the present study shows that miR-709 does not repress the same pool of genes in separate cell types. These results underscore the need for validating gene targets in every tissue a miRNA is expressed.

Original languageEnglish (US)
Article number18958
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 8 2016

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MicroRNAs
Cytoskeleton
Genes
Hepatocytes
Gene Pool
Untranslated RNA
Gene Regulatory Networks
Gene Expression Profiling
Cell Division
Extracellular Matrix
Cell Differentiation
Hepatocellular Carcinoma
Cell Death
Fatty Acids
Cell Proliferation
Gene Expression
Liver

ASJC Scopus subject areas

  • General

Cite this

Surendran, S., Jideonwo, V. N., Merchun, C., Ahn, M., Murray, J., Ryan, J., ... Morral, N. (2016). Gene targets of mouse miR-709: Regulation of distinct pools. Scientific Reports, 6, [18958]. https://doi.org/10.1038/srep18958

Gene targets of mouse miR-709 : Regulation of distinct pools. / Surendran, Sneha; Jideonwo, Victoria N.; Merchun, Chris; Ahn, Miwon; Murray, John; Ryan, Jennifer; Dunn, Kenneth; Kota, Janaiah; Morral, Nuria.

In: Scientific Reports, Vol. 6, 18958, 08.01.2016.

Research output: Contribution to journalArticle

Surendran, S, Jideonwo, VN, Merchun, C, Ahn, M, Murray, J, Ryan, J, Dunn, K, Kota, J & Morral, N 2016, 'Gene targets of mouse miR-709: Regulation of distinct pools', Scientific Reports, vol. 6, 18958. https://doi.org/10.1038/srep18958
Surendran S, Jideonwo VN, Merchun C, Ahn M, Murray J, Ryan J et al. Gene targets of mouse miR-709: Regulation of distinct pools. Scientific Reports. 2016 Jan 8;6. 18958. https://doi.org/10.1038/srep18958
Surendran, Sneha ; Jideonwo, Victoria N. ; Merchun, Chris ; Ahn, Miwon ; Murray, John ; Ryan, Jennifer ; Dunn, Kenneth ; Kota, Janaiah ; Morral, Nuria. / Gene targets of mouse miR-709 : Regulation of distinct pools. In: Scientific Reports. 2016 ; Vol. 6.
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