Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis

Taeok Bae, Elizabeth M. Class, Olaf Schneewind, Dominique Missiakas

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations

Abstract

Staphylococcus aureus is the leading cause of wound and hospital-acquired infections. The emergence of strains with resistance to all antibiotics has created a serious public health problem. Transposon-based mutagenesis can be used to generate libraries of mutants and to query genomes for factors involved in nonessential pathways, such as virulence and antibiotic resistance. Ideally, such studies should employ defined and complete sets of isogenic mutants and should be conducted so as to permit acquisition and comparison of the complete data sets. Such systematic knowledge can reveal entire pathways and can be exploited for the rational design of therapies. The mariner-based transposon, bursa aurealis, can be used to generate random libraries of mutants in laboratory strains and clinical isolates of S. aureus. This chapter describes a procedure for isolating mutants and mapping the insertion sites on the chromosome.

Original languageEnglish (US)
Title of host publicationMicrobial Gene Essentiality Protocols and Bioinformatics
Pages103-116
Number of pages14
StatePublished - Dec 1 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume416
ISSN (Print)1064-3745

Keywords

  • Bursa aurealis
  • Himar 1 transposase
  • Mariner
  • Mutagenesis
  • Staplrylococcus aureus
  • Temperature sensitive
  • Transposon library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Bae, T., Class, E. M., Schneewind, O., & Missiakas, D. (2007). Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. In Microbial Gene Essentiality Protocols and Bioinformatics (pp. 103-116). (Methods in Molecular Biology; Vol. 416).