Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis

Taeok Bae, Elizabeth M. Class, Olaf Schneewind, Dominique Missiakas

Research output: Chapter in Book/Report/Conference proceedingChapter

32 Citations (Scopus)

Abstract

Staphylococcus aureus is the leading cause of wound and hospital-acquired infections. The emergence of strains with resistance to all antibiotics has created a serious public health problem. Transposon-based mutagenesis can be used to generate libraries of mutants and to query genomes for factors involved in nonessential pathways, such as virulence and antibiotic resistance. Ideally, such studies should employ defined and complete sets of isogenic mutants and should be conducted so as to permit acquisition and comparison of the complete data sets. Such systematic knowledge can reveal entire pathways and can be exploited for the rational design of therapies. The mariner-based transposon, bursa aurealis, can be used to generate random libraries of mutants in laboratory strains and clinical isolates of S. aureus. This chapter describes a procedure for isolating mutants and mapping the insertion sites on the chromosome.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages103-116
Number of pages14
Volume416
StatePublished - Dec 1 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume416
ISSN (Print)10643745

Fingerprint

Insertional Mutagenesis
Libraries
Staphylococcus aureus
Genome
Microbial Drug Resistance
Cross Infection
Mutagenesis
Virulence
Public Health
Chromosomes
Anti-Bacterial Agents
Wounds and Injuries
Therapeutics
Datasets

Keywords

  • Bursa aurealis
  • Himar 1 transposase
  • Mariner
  • Mutagenesis
  • Staplrylococcus aureus
  • Temperature sensitive
  • Transposon library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bae, T., Class, E. M., Schneewind, O., & Missiakas, D. (2007). Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. In Methods in Molecular Biology (Vol. 416, pp. 103-116). (Methods in Molecular Biology; Vol. 416).

Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. / Bae, Taeok; Class, Elizabeth M.; Schneewind, Olaf; Missiakas, Dominique.

Methods in Molecular Biology. Vol. 416 2007. p. 103-116 (Methods in Molecular Biology; Vol. 416).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bae, T, Class, EM, Schneewind, O & Missiakas, D 2007, Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. in Methods in Molecular Biology. vol. 416, Methods in Molecular Biology, vol. 416, pp. 103-116.
Bae T, Class EM, Schneewind O, Missiakas D. Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. In Methods in Molecular Biology. Vol. 416. 2007. p. 103-116. (Methods in Molecular Biology).
Bae, Taeok ; Class, Elizabeth M. ; Schneewind, Olaf ; Missiakas, Dominique. / Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis. Methods in Molecular Biology. Vol. 416 2007. pp. 103-116 (Methods in Molecular Biology).
@inbook{48b451984f0642108e8c0347735b2c47,
title = "Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis",
abstract = "Staphylococcus aureus is the leading cause of wound and hospital-acquired infections. The emergence of strains with resistance to all antibiotics has created a serious public health problem. Transposon-based mutagenesis can be used to generate libraries of mutants and to query genomes for factors involved in nonessential pathways, such as virulence and antibiotic resistance. Ideally, such studies should employ defined and complete sets of isogenic mutants and should be conducted so as to permit acquisition and comparison of the complete data sets. Such systematic knowledge can reveal entire pathways and can be exploited for the rational design of therapies. The mariner-based transposon, bursa aurealis, can be used to generate random libraries of mutants in laboratory strains and clinical isolates of S. aureus. This chapter describes a procedure for isolating mutants and mapping the insertion sites on the chromosome.",
keywords = "Bursa aurealis, Himar 1 transposase, Mariner, Mutagenesis, Staplrylococcus aureus, Temperature sensitive, Transposon library",
author = "Taeok Bae and Class, {Elizabeth M.} and Olaf Schneewind and Dominique Missiakas",
year = "2007",
month = "12",
day = "1",
language = "English",
isbn = "9781597453219",
volume = "416",
series = "Methods in Molecular Biology",
pages = "103--116",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis

AU - Bae, Taeok

AU - Class, Elizabeth M.

AU - Schneewind, Olaf

AU - Missiakas, Dominique

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Staphylococcus aureus is the leading cause of wound and hospital-acquired infections. The emergence of strains with resistance to all antibiotics has created a serious public health problem. Transposon-based mutagenesis can be used to generate libraries of mutants and to query genomes for factors involved in nonessential pathways, such as virulence and antibiotic resistance. Ideally, such studies should employ defined and complete sets of isogenic mutants and should be conducted so as to permit acquisition and comparison of the complete data sets. Such systematic knowledge can reveal entire pathways and can be exploited for the rational design of therapies. The mariner-based transposon, bursa aurealis, can be used to generate random libraries of mutants in laboratory strains and clinical isolates of S. aureus. This chapter describes a procedure for isolating mutants and mapping the insertion sites on the chromosome.

AB - Staphylococcus aureus is the leading cause of wound and hospital-acquired infections. The emergence of strains with resistance to all antibiotics has created a serious public health problem. Transposon-based mutagenesis can be used to generate libraries of mutants and to query genomes for factors involved in nonessential pathways, such as virulence and antibiotic resistance. Ideally, such studies should employ defined and complete sets of isogenic mutants and should be conducted so as to permit acquisition and comparison of the complete data sets. Such systematic knowledge can reveal entire pathways and can be exploited for the rational design of therapies. The mariner-based transposon, bursa aurealis, can be used to generate random libraries of mutants in laboratory strains and clinical isolates of S. aureus. This chapter describes a procedure for isolating mutants and mapping the insertion sites on the chromosome.

KW - Bursa aurealis

KW - Himar 1 transposase

KW - Mariner

KW - Mutagenesis

KW - Staplrylococcus aureus

KW - Temperature sensitive

KW - Transposon library

UR - http://www.scopus.com/inward/record.url?scp=40549132515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40549132515&partnerID=8YFLogxK

M3 - Chapter

SN - 9781597453219

VL - 416

T3 - Methods in Molecular Biology

SP - 103

EP - 116

BT - Methods in Molecular Biology

ER -