Generation and characterization of Csrp1 enhancer-driven tissue-restricted Cre-recombinase mice

Paige Snider, Jana L. Fix, Rhonda Rogers, Goldie Peabody-Dowling, David Ingram, Brenda Lilly, Simon J. Conway

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Cell type-specific genetic modification using the LoxP/Cre system is a powerful tool for genetic analysis of distinct cell lineages. Because of the unique arterial smooth muscle-restricted expression of a 5.0 kb cysteine-rich protein (Csrp1) enhancer (Lilly et al., 2001, Dev Biol 240:531-547), we hypothesized that a transgenic Cre line would prove useful for the smooth muscle lineage-specific genetic manipulation. Here we describe a transgenic mouse line, ECsrp1Cre, where Cre is initially specifically expressed in arterial smooth muscle cells. Use of the ROSA26R reporter allele confirmed that Cre-mediated recombination in vascular smooth muscle cells began at ∼E10.0 and was highly proficient. Subsequently, Cre is expressed in restricted skeletal and non-vascular smooth muscle lineages. This lineage tracing data is important for future conditional knockout studies to understand where and when Cre-mediated deletion occurs and where Cre-expressing daughter cells finally localize. Additionally, we crossed the ECsrp1Cre mice to the ROSA26-eGFP-DTA diphtheria toxin A-expressing mice to genetically ablate ECsrp1Cre expressing cells. This ECsrp1Cre transgenic line should thus prove useful for genetic analysis of diverse aspects of cardiovascular morphogenesis and as a general smooth muscle lineage deletor line.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalGenesis
Volume46
Issue number3
DOIs
StatePublished - Mar 1 2008

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Keywords

  • Cysteine-rich protein-1
  • ECsrp1
  • Genetic cell ablation
  • Lineage mapping
  • Mouse embryo
  • Smooth muscle
  • Vascular remodeling

ASJC Scopus subject areas

  • Genetics

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